Literature DB >> 8391024

Detection of aquareovirus RNA in fish tissues by nucleic acid hybridization with a cloned cDNA probe.

K Subramanian1, B Lupiani, F M Hetrick, S K Samal.   

Abstract

A nucleic acid hybridization assay was developed to rapidly detect small quantities of aquareovirus RNAs in infected cells and organs. Cloned cDNA copies were synthesized from the genomic RNA of the SBR strain of aquareovirus. By using cloned cDNA probes, aquareovirus RNAs were detected in spleen and kidney tissues of experimentally infected fish.

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Year:  1993        PMID: 8391024      PMCID: PMC265588          DOI: 10.1128/jcm.31.6.1612-1614.1993

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  5 in total

1.  A simple and very efficient method for generating cDNA libraries.

Authors:  U Gubler; B J Hoffman
Journal:  Gene       Date:  1983-11       Impact factor: 3.688

2.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

3.  Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene.

Authors:  L W Cashdollar; J Esparza; G R Hudson; R Chmelo; P W Lee; W K Joklik
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205

4.  Sequence relatedness of Palyam virus genes to cognates of the Palyam serogroup viruses by RNA-RNA blot hybridization.

Authors:  D K Bodkin; D L Knudson
Journal:  Virology       Date:  1985-05       Impact factor: 3.616

5.  Heterogeneity in the genome RNAs and polypeptides of five members of a novel group of rotavirus-like viruses isolated from aquatic animals.

Authors:  S K Samal; C P Dopazo; K Subramanian; B Lupiani; S B Mohanty; F M Hetrick
Journal:  J Gen Virol       Date:  1991-01       Impact factor: 3.891

  5 in total
  1 in total

1.  Antibodies against outer-capsid proteins of grass carp reovirus expressed in E. coli are capable of neutralizing viral infectivity.

Authors:  Ling Shao; Xiaoyun Sun; Qin Fang
Journal:  Virol J       Date:  2011-07-12       Impact factor: 4.099

  1 in total

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