Literature DB >> 8390245

Cloning and functional expression of a human neuroendocrine vasoactive intestinal peptide receptor.

S P Sreedharan1, D R Patel, J X Huang, E J Goetzl.   

Abstract

Vasoactive intestinal peptide (VIP) is a potent mediator of gastrointestinal, nervous, pulmonary, vascular and immune functions. A cDNA was obtained from human HT29 intestinal epithelial cells and found to encode a 457 amino acid, 52 kDa VIP receptor. Transfection of the cDNA into COS-7 cells and 293 cells resulted in expression of specific saturable binding of VIP with a Kd of 0.8 nM, and induction of increases in intracellular cAMP by VIP with an EC50 of 1 nM. The human VIP receptor is homologous to other G protein-coupled receptors of the secretin-parathyroid hormone receptor family. A 2.8 kb transcript was detected in human lung, HT29 cells and Raji B-lymphoblasts with weaker expression in human brain, heart, kidney, liver and placenta.

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Year:  1993        PMID： 8390245     DOI： 10.1006/bbrc.1993.1658

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  20 in total

1.  Evolutionary relationships among G protein-coupled receptors using a clustered database approach.

Authors:  R C Graul; W Sadée
Journal:  AAPS PharmSci       Date:  2001

Review 2.  A VIP hybrid antagonist: from developmental neurobiology to clinical applications.

Authors:  I Gozes; M Fridkin; D E Brenneman
Journal:  Cell Mol Neurobiol       Date:  1995-12       Impact factor: 5.046

3.  Predominant expression of type II vasoactive intestinal peptide receptors by human T lymphoblastoma cells: transduction of both Ca2+ and cyclic AMP signals.

Authors:  M Xia; S P Sreedharan; E J Goetzl
Journal:  J Clin Immunol       Date:  1996-01       Impact factor: 8.317

4.  Identification of VIP/PACAP receptors on rat astrocytes using antisense oligodeoxynucleotides.

Authors:  O Ashur-Fabian; E Giladi; D E Brenneman; I Gozes
Journal:  J Mol Neurosci       Date:  1997-12       Impact factor: 3.444

5.  Effect of vasoactive intestinal peptide (VIP)-related peptides on cholinergic neurogenic and direct mucus secretion in ferret trachea in vitro.

Authors:  Y C Liu; A M Khawaja; D F Rogers
Journal:  Br J Pharmacol       Date:  1999-11       Impact factor: 8.739

6.  Vasoactive intestinal peptide prevents excitotoxic cell death in the murine developing brain.

Authors:  P Gressens; S Marret; J M Hill; D E Brenneman; I Gozes; M Fridkin; P Evrard
Journal:  J Clin Invest       Date:  1997-07-15       Impact factor: 14.808

7.  Structure, expression, and chromosomal localization of the type I human vasoactive intestinal peptide receptor gene.

Authors:  S P Sreedharan; J X Huang; M C Cheung; E J Goetzl
Journal:  Proc Natl Acad Sci U S A       Date:  1995-03-28       Impact factor: 11.205

8.  Expression and fine mapping of murine vasoactive intestinal peptide receptor 1.

Authors:  B Karacay; M S O'Dorisio; K Kasow; C Hollenback; R Krahe
Journal:  J Mol Neurosci       Date:  2001-12       Impact factor: 3.444

9.  Severe microcephaly induced by blockade of vasoactive intestinal peptide function in the primitive neuroepithelium of the mouse.

Authors:  P Gressens; J M Hill; B Paindaveine; I Gozes; M Fridkin; D E Brenneman
Journal:  J Clin Invest       Date:  1994-11       Impact factor: 14.808

10.  Molecular cloning and characterization of two pig vasoactive intestinal polypeptide receptors (VPAC1-R and VPAC2-R).

Authors:  Xiaping He; Fengyan Meng; Yajun Wang; Juan Li
Journal:  DNA Cell Biol       Date:  2014-02-12       Impact factor: 3.311
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