Sequence analysis of three non structural proteins of a porcine group C (Cowden strain) rotavirus.

Sequences of three gene products of a group C (Cowden strain) rotavirus are presented and compared with the sequences of the corresponding group A (SA11) proteins. The degree of similarity for gene 7, 9, and 10 is respectively 34%, 58%, and 45%. Comparison of these 2 viruses allowed to identify several regions well conserved. In the protein coded by Cowden segment 7 (NS 53) only a short cystein and histidine rich region, presenting the zinc finger consensus motif, is common to group A (segment 5) and group C sequences. Conversely the protein coded by segment 9 (NS 35) presented marked homology with group A NS 35. The protein coded by segment 10 (NS 26) is serine rich and presents an accumulation of charged residues near the carboxy terminus, like group A counterpart. This genomic segment presented a single large open reading frame, that contrasts with the group A counterpart for which a second out of phase ORF is used in rotavirus infected MA 104 cells.