Literature DB >> 8387187

Pituitary adenylate cyclase activating polypeptide receptors are present on small cell lung cancer cells.

T W Moody1, F Zia, A Makheja.   

Abstract

The effect of pituitary adenylate cyclase activating polypeptide (PACAP) on small cell lung cancer (SCLC) cells was investigated. In Fura 2-AM loaded NCI-N417 cells, PACAP-27 or PACAP-38 elevated cytosolic calcium in a dose-dependent manner. The cytosolic calcium was elevated from 133 to 185 nM with PACAP-27 (100 nM). Because PACAP-27 strongly elevated cytosolic calcium after the addition of 1 mM EGTA, PACAP released calcium from intracellular pools using NCI-N417 cells; similar results were obtained for SCLC cell line NCI-H345. Pituitary adenylate cyclase activating polypeptide-27 inhibited 125I-VIP and 125I-PACAP binding to NCI-N417 cells with high affinity (IC50 = 30 and 5 nM), whereas VIP inhibited 125I-VIP and 125I-PACAP-27 binding with IC50 values of 5 and 200 nM. Both 100 nM VIP and PACAP-27 elevated cAMP levels in NCI-H345 cells. In contrast, 1 microM VIP had no effect on cytosolic calcium, whereas 100 nM PACAP-27 caused a strong calcium response. Both 100 nM VIP and 10 nM PACAP-27 significantly stimulated the clonal growth of NCI-H345 cells. These data suggest that biologically active VIP and PACAP receptors are present on SCLC cells.

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Year:  1993        PMID： 8387187     DOI： 10.1016/0196-9781(93)90036-g

Source DB:  PubMed          Journal:  Peptides        ISSN： 0196-9781            Impact factor:   3.750


  15 in total

1.  PACAP immunoreactivity in human malignant tumor samples and cardiac diseases.

Authors:  Z Szanto; Zs Sarszegi; D Reglodi; J Nemeth; K Szabadfi; P Kiss; A Varga; E Banki; K Csanaky; B Gaszner; O Pinter; Zs Szalai; A Tamas
Journal:  J Mol Neurosci       Date:  2012-05-31       Impact factor: 3.444

2.  Pituitary adenylate cyclase-activating polypeptide causes tyrosine phosphorylation of the epidermal growth factor receptor in lung cancer cells.

Authors:  Terry W Moody; Nauramy Osefo; Bernardo Nuche-Berenguer; Lisa Ridnour; David Wink; Robert T Jensen
Journal:  J Pharmacol Exp Ther       Date:  2012-03-02       Impact factor: 4.030

3.  Pituitary adenylate cyclase-activating polypeptide causes increased tyrosine phosphorylation of focal adhesion kinase and paxillin.

Authors:  Terry W Moody; Julius Leyton; Robert T Jensen
Journal:  J Mol Neurosci       Date:  2011-09-06       Impact factor: 3.444

4.  Vasoactive intestinal peptide inhibits human small-cell lung cancer proliferation in vitro and in vivo.

Authors:  K Maruno; A Absood; S I Said
Journal:  Proc Natl Acad Sci U S A       Date:  1998-11-24       Impact factor: 11.205

5.  Differential coupling of the PAC1 SV1 splice variant on human colonic tumors to the activation of intracellular cAMP but not intracellular Ca2+ does not activate tumor proliferation.

Authors:  Patrizia M Germano; Sang V Le; David S Oh; Robert Fan; Sandy Lieu; Alan Siu; Joseph R Pisegna
Journal:  J Mol Neurosci       Date:  2004       Impact factor: 3.444

6.  Examination of PACAP-Like Immunoreactivity in Urogenital Tumor Samples.

Authors:  Andrea Tamas; Andras Javorhazy; Dora Reglodi; Donat Peter Sarlos; Daniel Banyai; David Semjen; Jozsef Nemeth; Beata Lelesz; Daniel Balazs Fulop; Zalan Szanto
Journal:  J Mol Neurosci       Date:  2015-10-10       Impact factor: 3.444

7.  Role of PACAP1 receptor in regulation of ECL cells and gastric acid secretion by pituitary adenylate cyclase activating peptide.

Authors:  J R Pisegna; G V Ohning; C Athmann; N Zeng; J H Walsh; G Sachs
Journal:  Ann N Y Acad Sci       Date:  2000       Impact factor: 5.691

8.  PAC1 and PACAP expression, signaling, and effect on the growth of HCT8, human colonic tumor cells.

Authors:  Sang V Le; Dean J Yamaguchi; Craig A McArdle; Ken Tachiki; Joseph R Pisegna; Patrizia Germano
Journal:  Regul Pept       Date:  2002-11-15

9.  PACAP-27 tyrosine phosphorylates mitogen activated protein kinase and increases VEGF mRNAs in human lung cancer cells.

Authors:  Terry W Moody; Julius Leyton; Marchessini Casibang; Joseph Pisegna; Robert T Jensen
Journal:  Regul Pept       Date:  2002-11-15

10.  PACAP induces signaling and stimulation of 5-hydroxytryptamine release and growth in neuroendocrine tumor cells.

Authors:  Patrizia M Germano; Sandy N Lieu; Janjing Xue; Helen J Cooke; Fievos L Christofi; Yuxin Lu; Joseph R Pisegna
Journal:  J Mol Neurosci       Date:  2009-08-23       Impact factor: 3.444
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