Comparison of the inhibitory effect of ouabain and dihydroouabain on the Na(+)-K+ ATPase from frog skin.

1. The effects of ouabain and dihydroouabain (DHO) on Na(+)-K+ ATPase from frog skin were investigated. 2. Ouabain seemed to be a more effective inhibitor, reaching near maximum inhibition at 10(-5) M while DHO reached near maximum at 10(-3) M. 3. Both inhibitors acted within the first few minutes, but ouabain inhibited more strongly over virtually the whole 2 hr considered. 4. Pre-incubation of the enzyme with ATP or Na+ reduced the level of inhibition due to subsequent ouabain or DHO, but preincubation with K+ did not.