Literature DB >> 8386978

Design of primers for PCR amplification of highly variable genomes.

J Dopazo1, A Rodríguez, J C Sáiz, F Sobrino.   

Abstract

A program to aid in the search of primers for specific polymerase chain reaction (PCR) amplification of highly variable genomes is presented. It involves the derivation of variability profiles to identify optimal regions for PCR amplification, taking into account stability of DNA-primer hybrids. An application of the program to foot-and-mouth disease virus diagnosis is presented.

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Year:  1993        PMID: 8386978     DOI: 10.1093/bioinformatics/9.2.123

Source DB:  PubMed          Journal:  Comput Appl Biosci        ISSN: 0266-7061


  5 in total

1.  A mathematical model and a computerized simulation of PCR using complex templates.

Authors:  E Rubin; A A Levy
Journal:  Nucleic Acids Res       Date:  1996-09-15       Impact factor: 16.971

2.  Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequences.

Authors:  T M Rose; E R Schultz; J G Henikoff; S Pietrokovski; C M McCallum; S Henikoff
Journal:  Nucleic Acids Res       Date:  1998-04-01       Impact factor: 16.971

3.  Closest string with outliers.

Authors:  Christina Boucher; Bin Ma
Journal:  BMC Bioinformatics       Date:  2011-02-15       Impact factor: 3.169

4.  A method for determining the position and size of optimal sequence regions for phylogenetic analysis.

Authors:  M J Martin; F González-Candelas; F Sobrino; J Dopazo
Journal:  J Mol Evol       Date:  1995-12       Impact factor: 2.395

5.  Identification of regions in multiple sequence alignments thermodynamically suitable for targeting by consensus oligonucleotides: application to HIV genome.

Authors:  Olga V Matveeva; Brian T Foley; Vladimir A Nemtsov; Raymond F Gesteland; Senya Matsufuji; John F Atkins; Alexei Y Ogurtsov; Svetlana A Shabalina
Journal:  BMC Bioinformatics       Date:  2004-04-29       Impact factor: 3.169

  5 in total

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