| Literature DB >> 8386525 |
Y Fujita1, M Mynlieff, R T Dirksen, M S Kim, T Niidome, J Nakai, T Friedrich, N Iwabe, T Miyata, T Furuichi.
Abstract
The complete amino acid sequence of a rabbit brain calcium channel (BIII) has been deduced by cloning and sequencing the cDNA. The open reading frame encodes 2339 amino acids, which corresponds to an M(r) of 261,167. A phylogenetic tree representing evolutionary relationships indicates that BIII is grouped together with the other rabbit brain calcium channels, BI and BII, into a subfamily that is distinct from the dihydropyridine-sensitive L-type subfamily. Transient expression in cultured skeletal muscle myotubes derived from muscular dysgenic mice demonstrates that the BIII channel mediates an omega-conotoxin-sensitive calcium current with kinetics and voltage dependence like those previously reported for whole-cell N-type current. Cell-attached patch recordings, with isotonic barium as the charge carrier, revealed distinct single channels with an average slope conductance of 14.3 pS.Entities:
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Year: 1993 PMID： 8386525 DOI： 10.1016/0896-6273(93)90162-k
Source DB: PubMed Journal: Neuron ISSN： 0896-6273 Impact factor: 17.173