Literature DB >> 8385929

A new, highly sensitive method for the detection and quantification of penicillin-binding proteins.

M Galleni1, B Lakaye, S Lepage, M Jamin, I Thamm, B Joris, J M Frère.   

Abstract

A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the rapid detection of 0.2 pmol with the naked eye and 2 fmol with the help of an A.L.F. automatic DNA sequencer. Direct labelling can also be performed on whole bacterial cells.

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Year:  1993        PMID： 8385929      PMCID： PMC1132474          DOI： 10.1042/bj2910019

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  Penicillin-binding proteins and cell shape in E. coli.

Authors:  B G Spratt; A B Pardee
Journal:  Nature       Date:  1975-04-10       Impact factor: 49.962

Review 2.  Serine beta-lactamases and penicillin-binding proteins.

Authors:  J M Ghuysen
Journal:  Annu Rev Microbiol       Date:  1991       Impact factor: 15.500

3.  Automated DNA sequencing: ultrasensitive detection of fluorescent bands during electrophoresis.

Authors:  W Ansorge; B Sproat; J Stegemann; C Schwager; M Zenke
Journal:  Nucleic Acids Res       Date:  1987-06-11       Impact factor: 16.971

4.  A complementation analysis of the restriction and modification of DNA in Escherichia coli.

Authors:  H W Boyer; D Roulland-Dussoix
Journal:  J Mol Biol       Date:  1969-05-14       Impact factor: 5.469

Review 5.  Penicillin-sensitive enzymes in peptidoglycan biosynthesis.

Authors:  J M Frère; B Joris
Journal:  Crit Rev Microbiol       Date:  1985       Impact factor: 7.624

6.  Purification and characterization of the penicillin-binding protein that is the lethal target of penicillin in Bacillus megaterium and Bacillus licheniformis. Protein exchange and complex stability.

Authors:  H A Chase; P E Reynolds; J B Ward
Journal:  Eur J Biochem       Date:  1978-07-17

7.  The genetics of Bacillus licheniformis penicillinase: a preliminary analysis from studies on mutation and inter-strain and intra-strain transformations.

Authors:  D A Dubnau; M R Pollock
Journal:  J Gen Microbiol       Date:  1965-10

Review 8.  Penicillin-binding proteins of gram-negative bacteria.

Authors:  B G Spratt; K D Cromie
Journal:  Rev Infect Dis       Date:  1988 Jul-Aug

9.  Properties of the penicillin-binding proteins of Escherichia coli K12,.

Authors:  B G Spratt
Journal:  Eur J Biochem       Date:  1977-01

10.  Synthesis of a 125I-radiolabeled penicillin for penicillin-binding proteins studies.

Authors:  J M Masson; R Labia
Journal:  Anal Biochem       Date:  1983-01       Impact factor: 3.365
  10 in total
  16 in total

1.  Identification of a novel penicillin-binding protein from Helicobacter pylori.

Authors:  P Krishnamurthy; M H Parlow; J Schneider; S Burroughs; C Wickland; N B Vakil; B E Dunn; S H Phadnis
Journal:  J Bacteriol       Date:  1999-08       Impact factor: 3.490

2.  Differential responses of Escherichia coli cells expressing cytoplasmic domain mutants of penicillin-binding protein 1b after impairment of penicillin-binding proteins 1a and 3.

Authors:  C Chalut; X Charpentier; M H Remy; J M Masson
Journal:  J Bacteriol       Date:  2001-01       Impact factor: 3.490

3.  BOCILLIN FL, a sensitive and commercially available reagent for detection of penicillin-binding proteins.

Authors:  G Zhao; T I Meier; S D Kahl; K R Gee; L C Blaszczak
Journal:  Antimicrob Agents Chemother       Date:  1999-05       Impact factor: 5.191

4.  Specificity inversion of Ochrobactrum anthropi D-aminopeptidase to a D,D-carboxypeptidase with new penicillin binding activity by directed mutagenesis.

Authors:  Michaël Delmarcelle; Marie-Caroline Boursoit; Patrice Filée; Stéphane Lucius Baurin; Jean-Marie Frère; Bernard Joris
Journal:  Protein Sci       Date:  2005-09       Impact factor: 6.725

5.  Visualization of penicillin-binding proteins during sporulation of Streptomyces griseus.

Authors:  J Hao; K E Kendrick
Journal:  J Bacteriol       Date:  1998-04       Impact factor: 3.490

6.  Site-directed mutagenesis of the Actinomadura R39 DD-peptidase.

Authors:  G H Zhao; C Duez; S Lepage; C Forceille; N Rhazi; D Klein; J M Ghuysen; J M Frère
Journal:  Biochem J       Date:  1997-10-15       Impact factor: 3.857

7.  Topographical and functional investigation of Escherichia coli penicillin-binding protein 1b by alanine stretch scanning mutagenesis.

Authors:  F Lefèvre; M H Rémy; J M Masson
Journal:  J Bacteriol       Date:  1997-08       Impact factor: 3.490

8.  Competition of various beta-lactam antibiotics for the major penicillin-binding proteins of Helicobacter pylori: antibacterial activity and effects on bacterial morphology.

Authors:  C R DeLoney; N L Schiller
Journal:  Antimicrob Agents Chemother       Date:  1999-11       Impact factor: 5.191

9.  Digoxigenin-ampicillin conjugate for detection of penicillin-binding proteins by chemiluminescence.

Authors:  L M Weigel; J T Belisle; J D Radolf; M V Norgard
Journal:  Antimicrob Agents Chemother       Date:  1994-02       Impact factor: 5.191

10.  Synthesis, purification and kinetic properties of fluorescein-labelled penicillins.

Authors:  B Lakaye; C Damblon; M Jamin; M Galleni; S Lepage; B Joris; J Marchand-Brynaert; C Frydrych; J M Frere
Journal:  Biochem J       Date:  1994-05-15       Impact factor: 3.857
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