Literature DB >> 8385421

Assay of nucleoside diphosphate kinase in microtiter plates using a peroxidase-coupled method.

I Lascu1, K LeBlay, M L Lacombe, E Presecan, M Véron.   

Abstract

A sensitive assay for nucleoside diphosphate kinase which utilizes microtiter plates is described. ATP, formed in the reaction between dGTP and ADP, is trapped by the glycerol kinase reaction. A colored compound is generated by glycerol-3-phosphate oxidase and peroxidase. This assay is useful for testing a large number of samples generated by chromatographic techniques or for screening purposes.

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Year:  1993        PMID: 8385421     DOI: 10.1006/abio.1993.1075

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  2 in total

Review 1.  The catalytic mechanism of nucleoside diphosphate kinases.

Authors:  I Lascu; P Gonin
Journal:  J Bioenerg Biomembr       Date:  2000-06       Impact factor: 2.945

2.  Substrate specificity and nucleotides binding properties of NM23H2/nucleoside diphosphate kinase homolog from Plasmodium falciparum.

Authors:  Mahmoud Kandeel; Yukio Kitade
Journal:  J Bioenerg Biomembr       Date:  2010-08-17       Impact factor: 2.945

  2 in total

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