OBJECTIVE: To determine whether preeclampsia enhances superoxide generation of neutrophils. METHODS: Samples were obtained from 12 normal nonpregnant, 15 normal pregnant, and 11 preeclamptic women. The superoxide production of neutrophils was measured by the superoxide dismutase-inhibitable reduction of ferricytochrome C at 550-540 nm using a dual-wavelength spectrophotometer. RESULTS: N-formyl-methionyl-leucyl-phenylalanine (FMLP)-induced superoxide production of neutrophils was significantly higher in preeclamptics than in normal nonpregnant and pregnant subjects, whereas phorbol 12-myristate 13-acetate-induced superoxide production did not differ among them. When neutrophils prepared from normal nonpregnant women were preincubated with sera from the study groups, sera from preeclamptics enhanced the FMLP-induced superoxide production to about twice that of the other subjects. The serum-enhancing factor appeared to be a heat-labile protein, different from other known activators. CONCLUSION: Preeclampsia is characterized by the presence of a neutrophil activator that enhances superoxide production. This enhanced production may contribute to the pathophysiologic changes observed in preeclampsia.
OBJECTIVE: To determine whether preeclampsia enhances superoxide generation of neutrophils. METHODS: Samples were obtained from 12 normal nonpregnant, 15 normal pregnant, and 11 preeclamptic women. The superoxide production of neutrophils was measured by the superoxide dismutase-inhibitable reduction of ferricytochrome C at 550-540 nm using a dual-wavelength spectrophotometer. RESULTS: N-formyl-methionyl-leucyl-phenylalanine (FMLP)-induced superoxide production of neutrophils was significantly higher in preeclamptics than in normal nonpregnant and pregnant subjects, whereas phorbol 12-myristate 13-acetate-induced superoxide production did not differ among them. When neutrophils prepared from normal nonpregnant women were preincubated with sera from the study groups, sera from preeclamptics enhanced the FMLP-induced superoxide production to about twice that of the other subjects. The serum-enhancing factor appeared to be a heat-labile protein, different from other known activators. CONCLUSION: Preeclampsia is characterized by the presence of a neutrophil activator that enhances superoxide production. This enhanced production may contribute to the pathophysiologic changes observed in preeclampsia.
Authors: Andrei L Kindzelskii; Ji-Biao Huang; Tinnakorn Chaiworapongsa; Ryan M Fahmy; Yeon Mee Kim; Roberto Romero; Howard R Petty Journal: J Clin Invest Date: 2002-12 Impact factor: 14.808
Authors: Ming-Lin Zhu; Jin-Ping Zhao; Ning Cui; Victor H Gonçalves-Rizzi; Jose S Possomato-Vieira; Regina A Nascimento; Carlos A Dias-Junior Journal: J Huazhong Univ Sci Technolog Med Sci Date: 2017-12-21
Authors: Robin E Gandley; Jennifer Rohland; Yan Zhou; Eiji Shibata; Gail F Harger; Augustine Rajakumar; Valerian E Kagan; Nina Markovic; Carl A Hubel Journal: Hypertension Date: 2008-06-30 Impact factor: 10.190