Literature DB >> 8383530

Structural characterization of azurin from Pseudomonas aeruginosa and some of its methionine-121 mutants.

L M Murphy1, R W Strange, B G Karlsson, L G Lundberg, T Pascher, B Reinhammar, S S Hasnain.   

Abstract

Azurin from Pseudomonas aeruginosa and two mutants where the methionine ligand has been mutated have been studied in order to directly investigate the functional and structural significance of this ligand in the blue copper proteins. Reduction potentials, X-ray absorption fine structure (XAFS), electron paramagnetic resonance (EPR), and optical spectra are obtained in an attempt to provide a direct correlation between the spectrochemical properties and the immediate structure of this redox center.

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Year:  1993        PMID： 8383530     DOI： 10.1021/bi00059a013

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  6 in total

Review 1.  Three-dimensional structures of laccases.

Authors:  N Hakulinen; J Rouvinen
Journal:  Cell Mol Life Sci       Date:  2015-01-14       Impact factor: 9.261

Review 2.  Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.

Authors:  Jing Liu; Saumen Chakraborty; Parisa Hosseinzadeh; Yang Yu; Shiliang Tian; Igor Petrik; Ambika Bhagi; Yi Lu
Journal:  Chem Rev       Date:  2014-04-23       Impact factor: 60.622

Review 3.  Plastocyanin: structural and functional analysis.

Authors:  M R Redinbo; T O Yeates; S Merchant
Journal:  J Bioenerg Biomembr       Date:  1994-02       Impact factor: 2.945

4.  Transforming a blue copper into a red copper protein: engineering cysteine and homocysteine into the axial position of azurin using site-directed mutagenesis and expressed protein ligation.

Authors:  Kevin M Clark; Yang Yu; Nicholas M Marshall; Nathan A Sieracki; Mark J Nilges; Ninian J Blackburn; Wilfred A van der Donk; Yi Lu
Journal:  J Am Chem Soc       Date:  2010-07-28       Impact factor: 15.419

5.  Site-directed mutations in fungal laccase: effect on redox potential, activity and pH profile.

Authors:  F Xu; R M Berka; J A Wahleithner; B A Nelson; J R Shuster; S H Brown; A E Palmer; E I Solomon
Journal:  Biochem J       Date:  1998-08-15       Impact factor: 3.857

6.  Prediction of Reduction Potentials of Copper Proteins with Continuum Electrostatics and Density Functional Theory.

Authors:  Nicholas J Fowler; Christopher F Blanford; Jim Warwicker; Sam P de Visser
Journal:  Chemistry       Date:  2017-09-21       Impact factor: 5.236
  6 in total

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