Literature DB >> 8382677

Phosphorylation of the Na,K-ATPase by Ca,phospholipid-dependent and cAMP-dependent protein kinases. Mapping of the region phosphorylated by Ca,phospholipid-dependent protein kinase.

A V Chibalin1, O D Lopina, S P Petukhov, L A Vasilets.   

Abstract

Ca,phospholipid-dependent (PKC) and cAMP-dependent (PKA) protein kinases phosphorylate the alpha-subunit of the Na,K-ATPase from duck salt gland with the incorporation of 0.3 and 0.5 mol 32P/mol of alpha-subunit, respectively. PKA (in contrast to PKC) phosphorylates the alpha-subunit only in the presence of detergents. Limited tryptic digestion of the Na,K-ATPase phosphorylated by PKC demonstrates that 32P is incorporated into the N-terminal 41-kDa fragment of the alpha-subunit. Selective chymotrypsin cleavage of phosphorylated enzyme yields a 35-kDa radioactive fragment derived from the central region of the alpha-subunit molecule. These findings suggest that PKC phosphorylates the alpha-subunit of the Na,K-ATPase within the region restricted by C3 and T1 cleavage sites.

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Year:  1993        PMID： 8382677     DOI： 10.1007/bf00768069

Source DB:  PubMed          Journal:  J Bioenerg Biomembr        ISSN： 0145-479X            Impact factor:   2.945


  14 in total

1.  Kinetics of phosphorylation of Na+/K(+)-ATPase by protein kinase C.

Authors:  J M Lowndes; M Hokin-Neaverson; P J Bertics
Journal:  Biochim Biophys Acta       Date:  1990-04-09

2.  Tryptic and chymotryptic cleavage sites in sequence of alpha-subunit of (Na+ + K+)-ATPase from outer medulla of mammalian kidney.

Authors:  P L Jørgensen; J H Collins
Journal:  Biochim Biophys Acta       Date:  1986-09-11

3.  Phosphorylation of smooth muscle caldesmon by three protein kinases: implication for domain mapping.

Authors:  A V Vorotnikov; V P Shirinsky; N B Gusev
Journal:  FEBS Lett       Date:  1988-08-29       Impact factor: 4.124

4.  A three-step purification procedure for protein kinase C: characterization of the purified enzyme.

Authors:  G M Walton; P J Bertics; L G Hudson; T S Vedvick; G N Gill
Journal:  Anal Biochem       Date:  1987-03       Impact factor: 3.365

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Proteolytic cleavage as a tool for studying structure and conformation of pure membrane-bound Na+, K+-ATPase.

Authors:  P L Jørgensen; R A Farley
Journal:  Methods Enzymol       Date:  1988       Impact factor: 1.600

7.  [Na,K-ATPase from duck salt glands].

Authors:  A A Boldyrev; O D Lopina; I A Svinukhova
Journal:  Biokhimiia       Date:  1981-08

8.  The (Na,K)-ATPase of Friend erythroleukemia cells is phosphorylated near the ATP hydrolysis by an endogenous membrane-bound kinase.

Authors:  L Ling; L Cantley
Journal:  J Biol Chem       Date:  1984-04-10       Impact factor: 5.157

9.  Regulation of endogenous and expressed Na+/K+ pumps in Xenopus oocytes by membrane potential and stimulation of protein kinases.

Authors:  L A Vasilets; W Schwarz
Journal:  J Membr Biol       Date:  1992-01       Impact factor: 1.843

10.  Phosphorylation of the (Na,K)-ATPase by a plasma membrane-bound protein kinase in friend erythroleukemia cells.

Authors:  L A Yeh; L Ling; L English; L Cantley
Journal:  J Biol Chem       Date:  1983-05-25       Impact factor: 5.157
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  3 in total

1.  Protein kinase C phosphorylation of purified Na,K-ATPase: C-terminal phosphorylation sites at the alpha- and gamma-subunits close to the inner face of the plasma membrane.

Authors:  Yasser A Mahmmoud; Flemming Cornelius
Journal:  Biophys J       Date:  2002-04       Impact factor: 4.033

2.  Voltage-dependent inhibition of the sodium pump by external sodium: species differences and possible role of the N-terminus of the alpha-subunit.

Authors:  L A Vasilets; T Ohta; S Noguchi; M Kawamura; W Schwarz
Journal:  Eur Biophys J       Date:  1993       Impact factor: 1.733

3.  Glucagon stimulation of hepatic Na(+)-pump activity and alpha-subunit phosphorylation in rat hepatocytes.

Authors:  C J Lynch; K M McCall; Y C Ng; S A Hazen
Journal:  Biochem J       Date:  1996-02-01       Impact factor: 3.857
  3 in total

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