Literature DB >> 8382016

A specific immunoprecipitation assay for the protein kinase FA/glycogen synthase kinase 3.

J Van Lint1, R L Khandelwal, W Merlevede, J R Vandenheede.   

Abstract

A specific immunoprecipitation assay has been developed for the accurate determination of the kinase FA/GSK3 activity in crude cell preparations. The assay is based on the production and isolation of polyclonal peptide antibodies toward the C-terminus of the rat GSK3-alpha and GSK3-beta isoforms. The respective forms of the kinases are captured as active immunocomplexes with immobilized secondary antibodies and their kinase activity toward the synthetic peptide P-GS1 can be accurately measured. Immunoprecipitation with a mixture of the two peptide antibodies allows for the detection and estimation of the total kinase FA/GSK3 activity in cellular extracts, whereas the alpha-peptide antibody specifically detects and measures the GSK3-alpha isoform. The contribution of GSK3-beta in the total kinase FA/GSK3 activity of cell fractions can be calculated.

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Year:  1993        PMID： 8382016     DOI： 10.1006/abio.1993.1018

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  8 in total

1.  Mice lacking phosphatase PP2A subunit PR61/B'delta (Ppp2r5d) develop spatially restricted tauopathy by deregulation of CDK5 and GSK3beta.

Authors:  Justin V Louis; Ellen Martens; Peter Borghgraef; Caroline Lambrecht; Ward Sents; Sari Longin; Karen Zwaenepoel; Robert Pijnenborg; Isabelle Landrieu; Guy Lippens; Birgit Ledermann; Jürgen Götz; Fred Van Leuven; Jozef Goris; Veerle Janssens
Journal:  Proc Natl Acad Sci U S A       Date:  2011-04-11       Impact factor: 11.205

2.  Immunological and biochemical study on tissue and subcellular distributions of protein kinase FA (an activating factor of ATP.Mg-dependent protein phosphatase): a simplified and efficient procedure for high quantity purification from brain.

Authors:  J S Yu; S D Yang
Journal:  J Protein Chem       Date:  1993-12

3.  Reciprocal regulation of glycogen phosphorylase and glycogen synthase by insulin involving phosphatidylinositol-3 kinase and protein phosphatase-1 in HepG2 cells.

Authors:  N A Syed; R L Khandelwal
Journal:  Mol Cell Biochem       Date:  2000-08       Impact factor: 3.396

4.  Effects of diabetes, vanadium, and insulin on glycogen synthase activation in Wistar rats.

Authors:  Sabina Semiz; Chris Orvig; John H McNeill
Journal:  Mol Cell Biochem       Date:  2002-02       Impact factor: 3.396

5.  The mechanism by which epidermal growth factor inhibits glycogen synthase kinase 3 in A431 cells.

Authors:  Y Saito; J R Vandenheede; P Cohen
Journal:  Biochem J       Date:  1994-10-01       Impact factor: 3.857

6.  The inhibition of glycogen synthase kinase-3 by insulin or insulin-like growth factor 1 in the rat skeletal muscle cell line L6 is blocked by wortmannin, but not by rapamycin: evidence that wortmannin blocks activation of the mitogen-activated protein kinase pathway in L6 cells between Ras and Raf.

Authors:  D A Cross; D R Alessi; J R Vandenheede; H E McDowell; H S Hundal; P Cohen
Journal:  Biochem J       Date:  1994-10-01       Impact factor: 3.857

7.  Regulation of mitochondrial pyruvate dehydrogenase activity by tau protein kinase I/glycogen synthase kinase 3beta in brain.

Authors:  M Hoshi; A Takashima; K Noguchi; M Murayama; M Sato; S Kondo; Y Saitoh; K Ishiguro; T Hoshino; K Imahori
Journal:  Proc Natl Acad Sci U S A       Date:  1996-04-02       Impact factor: 11.205

8.  Multiple signalling pathways involved in the stimulation of fatty acid and glycogen synthesis by insulin in rat epididymal fat cells.

Authors:  S K Moule; N J Edgell; G I Welsh; T A Diggle; E J Foulstone; K J Heesom; C G Proud; R M Denton
Journal:  Biochem J       Date:  1995-10-15       Impact factor: 3.857
  8 in total

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