Literature DB >> 8381810

Detection of hepatitis C virus RNA: comparison of one-stage polymerase chain reaction (PCR) with nested-set PCR.

D R Gretch1, J J Wilson, R L Carithers, C dela Rosa, J H Han, L Corey.   

Abstract

We evaluated a new hepatitis C virus RNA assay based on one-stage PCR followed by liquid hybridization with an oligonucleotide probe and compared it with nested-set PCR. The one-stage and nested-set PCR assays had identical sensitivities in analytical experiments and showed 100% concordance when clinical specimens were used. One-stage PCR may be less prone to contamination than nested-set PCR.

Entities:  

Mesh：

Substances：

Year:  1993        PMID： 8381810      PMCID： PMC262751          DOI： 10.1128/jcm.31.2.289-291.1993

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  A long-term study of hepatitis C virus replication in non-A, non-B hepatitis.

Authors:  P Farci; H J Alter; D Wong; R H Miller; J W Shih; B Jett; R H Purcell
Journal:  N Engl J Med       Date:  1991-07-11       Impact factor: 91.245

3.  Hepatitis C viral RNA in serum of patients with chronic non-A, non-B hepatitis: detection by the polymerase chain reaction using multiple primer sets.

Authors:  K Cristiano; A M Di Bisceglie; J H Hoofnagle; S M Feinstone
Journal:  Hepatology       Date:  1991-07       Impact factor: 17.425

4.  Detection, semiquantitation, and genetic variation in hepatitis C virus sequences amplified from the plasma of blood donors with elevated alanine aminotransferase.

Authors:  P P Ulrich; J M Romeo; P K Lane; I Kelly; L J Daniel; G N Vyas
Journal:  J Clin Invest       Date:  1990-11       Impact factor: 14.808

5.  The 5'-terminal sequence of the hepatitis C virus genome.

Authors:  H Okamoto; S Okada; Y Sugiyama; S Yotsumoto; T Tanaka; H Yoshizawa; F Tsuda; Y Miyakawa; M Mayumi
Journal:  Jpn J Exp Med       Date:  1990-06

6.  Characterization of the terminal regions of hepatitis C viral RNA: identification of conserved sequences in the 5' untranslated region and poly(A) tails at the 3' end.

Authors:  J H Han; V Shyamala; K H Richman; M J Brauer; B Irvine; M S Urdea; P Tekamp-Olson; G Kuo; Q L Choo; M Houghton
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-01       Impact factor: 11.205

7.  Use of aminotransferase, hepatitis C antibody, and hepatitis C polymerase chain reaction RNA assays to establish the diagnosis of hepatitis C virus infection in a diagnostic virology laboratory.

Authors:  D Gretch; W Lee; L Corey
Journal:  J Clin Microbiol       Date:  1992-08       Impact factor: 5.948

8.  Detection of hepatitis C viral sequences in blood donations by "nested" polymerase chain reaction and prediction of infectivity.

Authors:  J A Garson; R S Tedder; M Briggs; P Tuke; J A Glazebrook; A Trute; D Parker; J A Barbara; M Contreras; S Aloysius
Journal:  Lancet       Date:  1990-06-16       Impact factor: 79.321

9.  Hepatitis C quantification and sequencing in blood products, haemophiliacs, and drug users.

Authors:  P Simmonds; L Q Zhang; H G Watson; S Rebus; E D Ferguson; P Balfe; G H Leadbetter; P L Yap; J F Peutherer; C A Ludlam
Journal:  Lancet       Date:  1990-12-15       Impact factor: 79.321

10.  Detection of hepatitis C viral sequences in non-A, non-B hepatitis.

Authors:  A J Weiner; G Kuo; D W Bradley; F Bonino; G Saracco; C Lee; J Rosenblatt; Q L Choo; M Houghton
Journal:  Lancet       Date:  1990-01-06       Impact factor: 79.321
View more
  11 in total

1.  In situ distribution of hepatitis C virus replicative-intermediate RNA in hepatic tissue and its correlation with liver disease.

Authors:  M Chang; A P Marquardt; B L Wood; O Williams; S J Cotler; S L Taylor; R L Carithers; D R Gretch
Journal:  J Virol       Date:  2000-01       Impact factor: 5.103

2.  Prospective multicenter clinical evaluation of AMPLICOR and COBAS AMPLICOR hepatitis C virus tests.

Authors:  F S Nolte; M W Fried; M L Shiffman; A Ferreira-Gonzalez; C T Garrett; E R Schiff; S J Polyak; D R Gretch
Journal:  J Clin Microbiol       Date:  2001-11       Impact factor: 5.948

3.  Hepatitis C virus detection by single-round PCR specific for the terminal 3' noncoding region.

Authors:  F Umlauft; D T Wong; P J Oefner; P A Underhill; R C Cheung; T L Wright; A A Kolykhalov; K Gruenewald; H B Greenberg
Journal:  J Clin Microbiol       Date:  1996-10       Impact factor: 5.948

4.  Use of a commercial PCR kit for detection of hepatitis C virus.

Authors:  K Seme; M Poljak
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1995-06       Impact factor: 3.267

5.  Single-step PCR in molecular diagnosis of hepatitis C virus infection.

Authors:  E Farma; E Boeri; P Bettini; C M Repetto; J McDermott; F B Lillo; O E Varnier
Journal:  J Clin Microbiol       Date:  1996-12       Impact factor: 5.948

6.  Vaccination of chimpanzees against infection by the hepatitis C virus.

Authors:  Q L Choo; G Kuo; R Ralston; A Weiner; D Chien; G Van Nest; J Han; K Berger; K Thudium; C Kuo
Journal:  Proc Natl Acad Sci U S A       Date:  1994-02-15       Impact factor: 11.205

7.  The prevalence of hepatitis C virus (HCV) in infants and children after liver transplantation.

Authors:  M J Nowicki; N Ahmad; J E Heubi; I K Kuramoto; B M Baroudy; W F Balistreri
Journal:  Dig Dis Sci       Date:  1994-10       Impact factor: 3.199

8.  Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis C virus.

Authors:  Mohammad Kargar; Ahzam Askari; Abbas Doosti; Sadegh Ghorbani-Dalini
Journal:  Indian J Virol       Date:  2012-03-25

9.  Multigene tracking of hepatitis C virus quasispecies after liver transplantation: correlation of genetic diversification in the envelope region with asymptomatic or mild disease patterns.

Authors:  D G Sullivan; J J Wilson; R L Carithers; J D Perkins; D R Gretch
Journal:  J Virol       Date:  1998-12       Impact factor: 5.103

10.  Evaluation of the detection of Borrelia burgdorferi DNA in urine samples by polymerase chain reaction.

Authors:  M Maiwald; C Stockinger; D Hassler; M von Knebel Doeberitz; H G Sonntag
Journal:  Infection       Date:  1995 May-Jun       Impact factor: 3.553
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.