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Literature DB >> 8381410

Expression of mouse furin in a Chinese hamster cell resistant to Pseudomonas exotoxin A and viruses complements the genetic lesion.

J M Moehring¹, N M Inocencio, B J Robertson, T J Moehring.

Abstract

RPE.40 is a strain of mutated CHO-K1 cells with elevated resistance to Pseudomonas exotoxin A, Sindbis virus, and Newcastle disease virus. Virus resistance is due to an inability to cleave precursor viral membrane glycoproteins and produce infectious virions. Transfection of RPE.40 cells with cDNA for mouse furin causes them to lose all resistance and become as sensitive as wild-type cells to the toxin and viruses. Transfection of RPE.40 cells with cDNA for the related yeast protease Kex2 reduces their resistance to the toxin and viruses, but does not completely eliminate it.

Entities: Chemical

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Year: 1993 PMID： 8381410

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.486

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Cited

51 in total

1. Deletions in the transmembrane domain of a sindbis virus glycoprotein alter virus infectivity, stability, and host range.

Authors: Raquel Hernandez; Christine Sinodis; Michelle Horton; Davis Ferreira; Chunning Yang; Dennis T Brown
Journal: J Virol Date: 2003-12 Impact factor: 5.103

6. Inclusion of a furin-sensitive spacer enhances the cytotoxicity of ribotoxin restrictocin containing recombinant single-chain immunotoxins.

Authors: A Goyal; J K Batra
Journal: Biochem J Date: 2000-01-15 Impact factor: 3.857

Review 7. Furin: a mammalian subtilisin/Kex2p-like endoprotease involved in processing of a wide variety of precursor proteins.

Authors: K Nakayama
Journal: Biochem J Date: 1997-11-01 Impact factor: 3.857

Expression of mouse furin in a Chinese hamster cell resistant to Pseudomonas exotoxin A and viruses complements the genetic lesion.

1. Deletions in the transmembrane domain of a sindbis virus glycoprotein alter virus infectivity, stability, and host range.

2. Antibody to the E3 glycoprotein protects mice against lethal venezuelan equine encephalitis virus infection.

3. Location and role of free cysteinyl residues in the Sindbis virus E1 and E2 glycoproteins.

4. A role for PACE4 in the proteolytic activation of anthrax toxin protective antigen.

5. Requirement for presenilin 1 in facilitating lagged 2-mediated endoproteolysis and signaling of notch 1.

6. Inclusion of a furin-sensitive spacer enhances the cytotoxicity of ribotoxin restrictocin containing recombinant single-chain immunotoxins.

Review 7. Furin: a mammalian subtilisin/Kex2p-like endoprotease involved in processing of a wide variety of precursor proteins.

8. A furin-defective cell line is able to process correctly the gp160 of human immunodeficiency virus type 1.

9. Differential processing of sindbis virus glycoprotein PE2 in cultured vertebrate and arthropod cells.

10. Molecular targeting of intracellular compartments specifically in cancer cells.