Literature DB >> 8379936

Branched-chain-amino-acid biosynthesis in plants: molecular cloning and characterization of the gene encoding acetohydroxy acid isomeroreductase (ketol-acid reductoisomerase) from Arabidopsis thaliana (thale cress).

R Dumas1, G Curien, R T DeRose, R Douce.   

Abstract

Towards the goal of gaining a better understanding of the molecular mechanisms controlling branched-chain-amino-acid biosynthesis in plants, we have isolated, sequenced and characterized a gene encoding acetohydroxy acid isomero-reductase (ketol-acid reductoisomerase) from Arabidopsis thaliana (thale cress). Comparison between the acetohydroxy acid isomeroreductase cDNA and the genomic sequence has allowed us to determine the exon structure of the coding region. The isolated acetohydroxy acid isomeroreductase gene is distributed over approx. 4.5 kbp and contains nine introns (79-347 bp). The transcriptional start site was found to be 52 bp upstream of the translational initiation site. Southern-blot analysis of A. thaliana genomic DNA shows that the acetohydroxy acid isomeroreductase is encoded by a single-copy gene.

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Year:  1993        PMID： 8379936      PMCID： PMC1134535          DOI： 10.1042/bj2940821

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  33 in total

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Authors:  S M Arfin; H E Umbarger
Journal:  J Biol Chem       Date:  1969-03-10       Impact factor: 5.157

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Authors:  R Breathnach; P Chambon
Journal:  Annu Rev Biochem       Date:  1981       Impact factor: 23.643

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Authors:  E M Shematek; S M Arfin; W F Diven
Journal:  Arch Biochem Biophys       Date:  1973-09       Impact factor: 4.013

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Authors:  P A Pattee
Journal:  J Bacteriol       Date:  1976-09       Impact factor: 3.490

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Journal:  J Biol Chem       Date:  1975-02-10       Impact factor: 5.157

9.  Structure and expression of a cyanobacterial ilvC gene encoding acetohydroxyacid isomeroreductase.

Authors:  S Rieble; S I Beale
Journal:  J Bacteriol       Date:  1992-12       Impact factor: 3.490

10.  Nucleotide sequence and characterization of a cDNA encoding the acetohydroxy acid isomeroreductase from Arabidopsis thaliana.

Authors:  G Curien; R Dumas; R Douce
Journal:  Plant Mol Biol       Date:  1993-02       Impact factor: 4.076
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  6 in total

1.  Branched-Chain Amino Acid Metabolism in Arabidopsis thaliana.

Authors:  Stefan Binder
Journal:  Arabidopsis Book       Date:  2010-08-23

2.  Cloning and expression of the pea gene encoding SBP65, a seed-specific biotinylated protein.

Authors:  L Dehaye; M Duval; D Viguier; J Yaxley; D Job
Journal:  Plant Mol Biol       Date:  1997-11       Impact factor: 4.076

3.  Towards identifying the full set of genes expressed during cassava post-harvest physiological deterioration.

Authors:  Kim Reilly; Diana Bernal; Diego F Cortés; Rocío Gómez-Vásquez; Joe Tohme; John R Beeching
Journal:  Plant Mol Biol       Date:  2007-02-22       Impact factor: 4.076

4.  A 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesis.

Authors:  S Takahashi; T Kuzuyama; H Watanabe; H Seto
Journal:  Proc Natl Acad Sci U S A       Date:  1998-08-18       Impact factor: 11.205

5.  General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH.

Authors:  Sabine Brinkmann-Chen; Tilman Flock; Jackson K B Cahn; Christopher D Snow; Eric M Brustad; John A McIntosh; Peter Meinhold; Liang Zhang; Frances H Arnold
Journal:  Proc Natl Acad Sci U S A       Date:  2013-06-17       Impact factor: 11.205

6.  Interactions of plant acetohydroxy acid isomeroreductase with reaction intermediate analogues: correlation of the slow, competitive, inhibition kinetics of enzyme activity and herbicidal effects.

Authors:  R Dumas; C Cornillon-Bertrand; P Guigue-Talet; P Genix; R Douce; D Job
Journal:  Biochem J       Date:  1994-08-01       Impact factor: 3.857
  6 in total

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