Liquid chromatography with electrochemical detection for quantitation of bound choline liberated by phospholipase D hydrolysis from phospholipids containing choline in rat plasma.

This report describes the optimal conditions for the determination of bound choline in rat plasma. The method used was based on the liberation of choline by phospholipase D from phospholipids containing choline in plasma, followed by high-performance liquid chromatographic analysis. Normal concentrations of total, free and bound choline in rat plasma were found to be 1278.7 +/- 132.5, 11.5 +/- 2.2 and 1267.2 +/- 125.5 nmol/ml, respectively. The described procedure has the advantages of rapidity, specificity, excellent precision and the need for only a small amount of the sample.