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Literature DB >> 8374957

An essential role for phosphatidylinositol transfer protein in phospholipase C-mediated inositol lipid signaling.

G M Thomas¹, E Cunningham, A Fensome, A Ball, N F Totty, O Truong, J J Hsuan, S Cockcroft.

Abstract

Transmembrane signaling by the phospholipase C-beta (PLC-beta) pathway is known to require at least three components: the receptor, the G protein, and the PLC. Recent studies have indicated that if the cytosol is allowed to leak out of HL60 cells, then G protein-stimulated PLC activity is greatly diminished, indicating an essential role for a cytosolic component(s). We now report the complete purification of one component based on its ability to reconstitute GTP gamma S-mediated PLC activity and identify it as the phosphatidylinositol transfer protein (PI-TP). Based on the in vitro effects of PI-TP, we surmise that it is involved in transporting PI from intracellular compartments for conversion to PI bisphosphate (PIP2) prior to hydrolysis by PLC-beta 2/PLC-beta 3, the endogenous PLC isoforms present in these cells.

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Year: 1993 PMID： 8374957 DOI： 10.1016/0092-8674(93)90471-2

Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582

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Cited

50 in total

1. Effects of NMDA on carbachol-stimulated phosphatidylinositol resynthesis in rat brain cortical slices.

Authors: H M Lee; C L Huang
Journal: Neurochem Res Date: 1997-05 Impact factor: 3.996

2. Identification and characterization of differentially active pools of type IIalpha phosphatidylinositol 4-kinase activity in unstimulated A431 cells.

Authors: Mark G Waugh; Shane Minogue; Deena Blumenkrantz; J Simon Anderson; J Justin Hsuan
Journal: Biochem J Date: 2003-12-01 Impact factor: 3.857

3. The yeast and mammalian isoforms of phosphatidylinositol transfer protein can all restore phospholipase C-mediated inositol lipid signaling in cytosol-depleted RBL-2H3 and HL-60 cells.

Authors: E Cunningham; S K Tan; P Swigart; J Hsuan; V Bankaitis; S Cockcroft
Journal: Proc Natl Acad Sci U S A Date: 1996-06-25 Impact factor: 11.205

4. Phosphatidylinositol- and phosphatidylcholine-transfer activity of PITPbeta is essential for COPI-mediated retrograde transport from the Golgi to the endoplasmic reticulum.

Authors: Nicolas Carvou; Roman Holic; Michelle Li; Clare Futter; Alison Skippen; Shamshad Cockcroft
Journal: J Cell Sci Date: 2010-03-23 Impact factor: 5.285

10. Cancer genomics identifies regulatory gene networks associated with the transition from dysplasia to advanced lung adenocarcinomas induced by c-Raf-1.

Authors: Astrid Rohrbeck; Jürgen Borlak
Journal: PLoS One Date: 2009-10-08 Impact factor: 3.240

An essential role for phosphatidylinositol transfer protein in phospholipase C-mediated inositol lipid signaling.

1. Effects of NMDA on carbachol-stimulated phosphatidylinositol resynthesis in rat brain cortical slices.

2. Identification and characterization of differentially active pools of type IIalpha phosphatidylinositol 4-kinase activity in unstimulated A431 cells.

3. The yeast and mammalian isoforms of phosphatidylinositol transfer protein can all restore phospholipase C-mediated inositol lipid signaling in cytosol-depleted RBL-2H3 and HL-60 cells.

4. Phosphatidylinositol- and phosphatidylcholine-transfer activity of PITPbeta is essential for COPI-mediated retrograde transport from the Golgi to the endoplasmic reticulum.

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9. Proteomic analysis of rat brains following exposure to electroconvulsive therapy.

10. Cancer genomics identifies regulatory gene networks associated with the transition from dysplasia to advanced lung adenocarcinomas induced by c-Raf-1.