Literature DB >> 8373596

Shadow bands seen when typing polymorphic dinucleotide repeats: some causes and cures.

M Litt1, X Hauge, V Sharma.   

Abstract

Polymorphic dinucleotide repeats are generally typed by using the PCR to generate products that are resolved on denaturing acrylamide gels. The presence of "shadow bands" on these gels often makes it difficult to score alleles reliably. We have developed procedures that overcome many of these difficulties. Important aspects of these improved procedures include using gels containing formamide as well as urea, transferring the resolved allelic fragments to nylon membranes by capillary blotting and probing the membranes with locus-specific oligonucleotide probes.

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Year:  1993        PMID： 8373596

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  31 in total

1.  Variability in the interpretation of microsatellite patterns with different electrophoretic conditions.

Authors:  A C Santos; Y Yamaoka; D Y Graham; A R Sepulveda
Journal:  Mol Pathol       Date:  1999-10

2.  Loss of heterozygosity analysis using whole genome amplification, cell sorting, and fluorescence-based PCR.

Authors:  T G Paulson; P C Galipeau; B J Reid
Journal:  Genome Res       Date:  1999-05       Impact factor: 9.043

3.  SCOMP is superior to degenerated oligonucleotide primed-polymerase chain reaction for global amplification of minute amounts of DNA from microdissected archival tissue samples.

Authors:  Nikolas H Stoecklein; Andreas Erbersdobler; Oleg Schmidt-Kittler; Joachim Diebold; Julian A Schardt; Jakob R Izbicki; Christoph A Klein
Journal:  Am J Pathol       Date:  2002-07       Impact factor: 4.307

4.  Precise assessment of microsatellite instability using high resolution fluorescent microsatellite analysis.

Authors:  S Oda; E Oki; Y Maehara; K Sugimachi
Journal:  Nucleic Acids Res       Date:  1997-09-01       Impact factor: 16.971

5.  Use of non-radioactive detection in SSCP, direct DNA sequencing and LOH analysis.

Authors:  I Petersen; M B Reichel; M Dietel
Journal:  Clin Mol Pathol       Date:  1996-04

6.  Quantitative trait locus mapping in dairy cattle by means of selective milk DNA pooling using dinucleotide microsatellite markers: analysis of milk protein percentage.

Authors:  E Lipkin; M O Mosig; A Darvasi; E Ezra; A Shalom; A Friedmann; M Soller
Journal:  Genetics       Date:  1998-07       Impact factor: 4.562

7.  Sequence analysis and characterization of stutter products at the tetranucleotide repeat locus vWA.

Authors:  P S Walsh; N J Fildes; R Reynolds
Journal:  Nucleic Acids Res       Date:  1996-07-15       Impact factor: 16.971

8.  Reliable genotyping of samples with very low DNA quantities using PCR.

Authors:  P Taberlet; S Griffin; B Goossens; S Questiau; V Manceau; N Escaravage; L P Waits; J Bouvet
Journal:  Nucleic Acids Res       Date:  1996-08-15       Impact factor: 16.971

9.  Mapping a gene for adult-onset primary open-angle glaucoma to chromosome 3q.

Authors:  M K Wirtz; J R Samples; P L Kramer; K Rust; J R Topinka; J Yount; R D Koler; T S Acott
Journal:  Am J Hum Genet       Date:  1997-02       Impact factor: 11.025

10.  Molecular characterization of two proximal deletion breakpoint regions in both Prader-Willi and Angelman syndrome patients.

Authors:  S L Christian; W P Robinson; B Huang; A Mutirangura; M R Line; M Nakao; U Surti; A Chakravarti; D H Ledbetter
Journal:  Am J Hum Genet       Date:  1995-07       Impact factor: 11.025
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