Literature DB >> 8373591

A rapid optimized protocol for downward alkaline Southern blotting of DNA.

P A Koetsier1, J Schorr, W Doerfler.   

Abstract

A simple and efficient 2.5-h Southern blotting procedure is described that uses 0.4 M NaOH to transfer DNA in a downward direction. The resulting blots give signals that are both sharper and 30% stronger than those obtained by conventional upward-transferred blots.

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Year:  1993        PMID： 8373591

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  43 in total

1.  Posttranscriptional gene silencing in transgenic sugarcane. Dissection Of homology-dependent virus resistance in a monocot that has a complex polyploid genome

Authors: 
Journal:  Plant Physiol       Date:  1999-04       Impact factor: 8.340

2.  Influence of in vitro manipulation on the stability of methylation patterns in the Snurf/Snrpn-imprinting region in mouse embryonic stem cells.

Authors:  Axel Schumacher; Walter Doerfler
Journal:  Nucleic Acids Res       Date:  2004-03-05       Impact factor: 16.971

3.  Ectopic expression of a conifer Abscisic Acid Insensitive3 transcription factor induces high-level synthesis of recombinant human alpha-L-iduronidase in transgenic tobacco leaves.

Authors:  Allison R Kermode; Ying Zeng; Xiaoke Hu; Samantha Lauson; Suzanne R Abrams; Xu He
Journal:  Plant Mol Biol       Date:  2007-01-04       Impact factor: 4.076

4.  Comparative physiological and molecular responses of a common aromatic indica rice cultivar to high salinity with non-aromatic indica rice cultivars.

Authors:  Aryadeep Roychoudhury; Supratim Basu; Sailendra N Sarkar; Dibyendu N Sengupta
Journal:  Plant Cell Rep       Date:  2008-05-29       Impact factor: 4.570

5.  Deletion of the Schizophyllum commune A alpha locus: the roles of A alpha Y and Z mating-type genes.

Authors:  C I Robertson; K A Bartholomew; C P Novotny; R C Ullrich
Journal:  Genetics       Date:  1996-12       Impact factor: 4.562

6.  Bronchioalveolar stem cells are a main source for regeneration of distal lung epithelia in vivo.

Authors:  Isabelle Salwig; Birgit Spitznagel; Ana Ivonne Vazquez-Armendariz; Keynoosh Khalooghi; Stefan Guenther; Susanne Herold; Marten Szibor; Thomas Braun
Journal:  EMBO J       Date:  2019-04-26       Impact factor: 11.598

7.  Significance of the expression of the CER6 condensing enzyme for cuticular wax production in Arabidopsis.

Authors:  Tanya S Hooker; Anthony A Millar; Ljerka Kunst
Journal:  Plant Physiol       Date:  2002-08       Impact factor: 8.340

8.  The structure of adenovirus type 12 DNA integration sites in the hamster cell genome.

Authors:  M Knoblauch; J Schröer; B Schmitz; W Doerfler
Journal:  J Virol       Date:  1996-06       Impact factor: 5.103

9.  Autocrine growth stimulation of SW403 colon carcinoma cell line is caused by transforming-growth-factor-alpha-mediated epidermal growth factor receptor activation.

Authors:  T Hirsch; S Eggstein; S Frank; E Farthmann; B U von Specht
Journal:  J Cancer Res Clin Oncol       Date:  1996       Impact factor: 4.553

10.  Insertion of foreign DNA into an established mammalian genome can alter the methylation of cellular DNA sequences.

Authors:  R Remus; C Kämmer; H Heller; B Schmitz; G Schell; W Doerfler
Journal:  J Virol       Date:  1999-02       Impact factor: 5.103
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