Literature DB >> 8370745

Evaluation of conventional media for detection of colonization factor antigens of enterotoxigenic Escherichia coli.

A R Ghosh1, D Sen, D A Sack, A T Hoque.   

Abstract

Strains of enterotoxigenic Escherichia coli producing either colonization factor antigen (CFA) I or II were tested for expression of CFA when grown on 16 different agar media by using agglutination and coagglutination with monoclonal antibodies, mannose-resistant hemagglutination, and a salt aggregation assay. CFA was detected from the CFA-positive strains when CFA agar was used, and it was also detected when other commercially available media were used, notably nutrient agar. CFA was not detected when other commercial media such as MacConkey agar were used. The use of nutrient agar with monoclonal antibody-based coagglutination reagents offers a potentially simple and rapid method for detecting E. coli which express CFA I or II.

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Year:  1993        PMID: 8370745      PMCID: PMC265715          DOI: 10.1128/jcm.31.8.2163-2166.1993

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  12 in total

1.  Rapid identification of colonization factor antigens (CFA/I and CFA/II) of enterotoxigenic E. coli (ETEC) and O-antigens of enteropathogenic E. coli (EPEC) by coagglutination test.

Authors:  A Faris; A Ljungh; T Wadström
Journal:  Zentralbl Bakteriol Mikrobiol Hyg A       Date:  1985-07

2.  Comparison of methods for detection of colonization factor antigens on enterotoxigenic Escherichia coli.

Authors:  C M Ahrén; L Gothefors; B J Stoll; M A Salek; A M Svennerholm
Journal:  J Clin Microbiol       Date:  1986-03       Impact factor: 5.948

3.  Mapping of a plasmid, coding for colonization, factor antigen I and heat-stable enterotoxin production, isolated from an enterotoxigenic strain of Escherichia coli.

Authors:  H R Smith; G A Willshaw; B Rowe
Journal:  J Bacteriol       Date:  1982-01       Impact factor: 3.490

4.  Monoclonal antibodies against the different subcomponents of colonization factor antigen II of enterotoxigenic Escherichia coli.

Authors:  Y Lopez-Vidal; A M Svennerholm
Journal:  J Clin Microbiol       Date:  1990-09       Impact factor: 5.948

5.  Hemadsorption and enzyme-linked immunosorbent assay nitrocellulose replica methods for identification of colonization factor antigen (CFA)-positive Escherichia coli colonies and for isolation of CFA-negative mutants.

Authors:  Y López-Vidal; A M Svennerholm
Journal:  J Clin Microbiol       Date:  1986-10       Impact factor: 5.948

6.  Characterization of plasmids that encode for the K88 colonization antigen.

Authors:  P L Shipley; C L Gyles; S Falkow
Journal:  Infect Immun       Date:  1978-05       Impact factor: 3.441

7.  Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor.

Authors:  D G Evans; D J Evans; W Tjoa
Journal:  Infect Immun       Date:  1977-11       Impact factor: 3.441

8.  Virulence factors of enterotoxigenic Escherichia coli.

Authors:  D G Evans; D J Evans; H L DuPont
Journal:  J Infect Dis       Date:  1977-08       Impact factor: 5.226

9.  Detection of enterotoxigenic Escherichia coli colonization factor antigen I in stool specimens by an enzyme-linked immunosorbent assay.

Authors:  D G Evans; D J Evans; S Clegg
Journal:  J Clin Microbiol       Date:  1980-12       Impact factor: 5.948

10.  Roles of different coli surface antigens of colonization factor antigen II in colonization by and protective immunogenicity of enterotoxigenic Escherichia coli in rabbits.

Authors:  A M Svennerholm; C Wennerås; J Holmgren; M M McConnell; B Rowe
Journal:  Infect Immun       Date:  1990-02       Impact factor: 3.441

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  3 in total

1.  Identification of Novel Components Influencing Colonization Factor Antigen I Expression in Enterotoxigenic Escherichia coli.

Authors:  Sara Haines; Sylviane Gautheron; William Nasser; Geneviève Renauld-Mongénie
Journal:  PLoS One       Date:  2015-10-30       Impact factor: 3.240

2.  Bioactive Immune Components of Anti-Diarrheagenic Enterotoxigenic Escherichia coli Hyperimmune Bovine Colostrum Products.

Authors:  Khandra T Sears; Sharon M Tennant; Mardi K Reymann; Raphael Simon; Nicky Konstantopoulos; William C Blackwelder; Eileen M Barry; Marcela F Pasetti
Journal:  Clin Vaccine Immunol       Date:  2017-08-04

Review 3.  Appraisal of microbial evolution to commensalism and pathogenicity in humans.

Authors:  Asit Ranjan Ghosh
Journal:  Clin Med Insights Gastroenterol       Date:  2013-05-27
  3 in total

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