| Literature DB >> 8368157 |
M Moser1, R Crameri, G Menz, M Suter.
Abstract
A. fumigatus allergen I/a (rAsp f I/a) was produced in Escherichia coli, affinity purified by Ni(2+)-chelate chromatography and refolded. rAsp f I/a binds IgE of A. fumigatus-allergic individuals as shown by ELISA, dot blots and Western blots. In addition, rAsp I/a elicited positive immediate type I skin reactions in individuals allergic to A. fumigatus but not in control individuals. Hence, rAsp f I/a can be used as a standardized antigen/allergen for an improved serological and clinical diagnosis of A. fumigatus-associated diseases.Entities:
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Year: 1993 PMID: 8368157 DOI: 10.1007/978-3-0348-7324-6_11
Source DB: PubMed Journal: Agents Actions Suppl ISSN: 0379-0363