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Literature DB >> 8367472

Recombination walking: genetic selection of clones from pooled libraries of yeast artificial chromosomes by homologous recombination.

A M Miller¹, E A Savinelli, S M Couture, G M Hannigan, Z Han, R F Selden, D A Treco.

Abstract

Recombination walking is based on the genetic selection of specific human clones from a yeast artificial chromosome (YAC) library by homologous recombination. The desired clone is selected from a pooled (unordered) YAC library, eliminating labor-intensive steps typically used in organizing and maintaining ordered YAC libraries. Recombination walking represents an efficient approach to library screening and is well suited for chromosome-walking approaches to the isolation of genes associated with common diseases.

Entities: Species

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Year: 1993 PMID： 8367472 PMCID： PMC47299 DOI： 10.1073/pnas.90.17.8118

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

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References

27 in total

1. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

Authors: A C Chang; S N Cohen
Journal: J Bacteriol Date: 1978-06 Impact factor: 3.490

Recombination walking: genetic selection of clones from pooled libraries of yeast artificial chromosomes by homologous recombination.

1. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

2. Systematic screening of yeast artificial-chromosome libraries by use of the polymerase chain reaction.

3. Improved genetic selection for screening bacteriophage libraries by homologous recombination in vivo.

4. Replacement of chromosome segments with altered DNA sequences constructed in vitro.

5. Polyamines eliminate an extreme size bias against transformation of large yeast artificial chromosome DNA.

6. An initiation site for meiotic gene conversion in the yeast Saccharomyces cerevisiae.

7. Isolation of single-copy human genes from a library of yeast artificial chromosome clones.

8. Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae.

9. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325.

10. Identification of the cystic fibrosis gene: chromosome walking and jumping.