Changes in oxidative metabolism of murine spleen following laser and superluminous diode (660-950 nm) irradiation: effects of cellular composition and radiation parameters.

Chemiluminescence (CL) of splenocytes of A/Sn mice was recorded after irradiation of the cells with various individual laser an superluminous diode probes at wavelengths from 660 to 950 nm (pulse repetition rates varying from 4 to 5,000 Hz) and at various doses. Laser radiation was found to increase or suppress the spontaneous CL of splenocytes suspension, the amplitude and the sign of the effect depending on the cellular composition of the samples. Direct correlations between the effect of laser radiation (per cent in changes of CL when irradiated at 820 nm, 1.1 x 10(3) J/m2, 292 Hz) and per cent of plasmacytes (r = 0.743, P < 0.001), neutrophils (r = 0.650, P < 0.001) as well as myelocytes and metamyelocytes (r = 0.505, P < 0.01) were established. The correlation with per cent of lymphocytes (r = -0.590, P < 0.001) was found to be a reverse one. Dependence of the irradiation effects on dose, pulse repetition rate, and wavelength are presented.