Literature DB >> 8366057

The chimeric VirA-tar receptor protein is locked into a highly responsive state.

S C Turk1, R P van Lange, E Sonneveld, P J Hooykaas.   

Abstract

The wild-type VirA protein is known to be responsive not only to phenolic compounds but also to sugars via the ChvE protein (G. A. Cangelosi, R. G. Ankenbauer, and E. W. Nester, Proc. Natl. Acad. Sci. USA 87:6708-6712, 1990, and N. Shimoda, A. Toyoda-Yamamoto, J. Nagamine, S. Usami, M. Katayama, Y. Sakagami, and Y. Machida, Proc. Natl. Acad. Sci. USA 87:6684-6688, 1990). It is shown here that the mutant VirA(Ser-44, Arg-45) protein and the chimeric VirA-Tar protein are no longer responsive to sugars and the ChvE protein. However, whereas the chimeric VirA-Tar protein was found to be locked in a highly responsive state, the VirA(Ser-44, Arg-45) mutant protein appeared to be locked in a low responsive state. This difference turned out to be important for tumorigenicity of the host strains in virulence assays on Kalanchoë daigremontiana.

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Year:  1993        PMID： 8366057      PMCID： PMC206631          DOI： 10.1128/jb.175.17.5706-5709.1993

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  24 in total

1.  Characterization of the VirG binding site of Agrobacterium tumefaciens.

Authors:  G J Pazour; A Das
Journal:  Nucleic Acids Res       Date:  1990-12-11       Impact factor: 16.971

2.  Fine-tuning the topology of a polytopic membrane protein: role of positively and negatively charged amino acids.

Authors:  I Nilsson; G von Heijne
Journal:  Cell       Date:  1990-09-21       Impact factor: 41.582

Review 3.  Agrobacterium and plant genetic engineering.

Authors:  P J Hooykaas; R A Schilperoort
Journal:  Plant Mol Biol       Date:  1992-05       Impact factor: 4.076

4.  Signal structure for transcriptional activation in the upstream regions of virulence genes on the hairy-root-inducing plasmid A4.

Authors:  T Aoyama; M Takanami; A Oka
Journal:  Nucleic Acids Res       Date:  1989-11-11       Impact factor: 16.971

5.  Sugars induce the Agrobacterium virulence genes through a periplasmic binding protein and a transmembrane signal protein.

Authors:  G A Cangelosi; R G Ankenbauer; E W Nester
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

6.  Conserved domains in bacterial regulatory proteins that respond to environmental stimuli.

Authors:  C W Ronson; B T Nixon; F M Ausubel
Journal:  Cell       Date:  1987-06-05       Impact factor: 41.582

7.  The regulatory VirG protein specifically binds to a cis-acting regulatory sequence involved in transcriptional activation of Agrobacterium tumefaciens virulence genes.

Authors:  S G Jin; T Roitsch; P J Christie; E W Nester
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

8.  The virA promoter is a host-range determinant in Agrobacterium tumefaciens.

Authors:  S C Turk; E W Nester; P J Hooykaas
Journal:  Mol Microbiol       Date:  1993-03       Impact factor: 3.501

9.  virA and virG control the plant-induced activation of the T-DNA transfer process of A. tumefaciens.

Authors:  S E Stachel; P C Zambryski
Journal:  Cell       Date:  1986-08-01       Impact factor: 41.582

10.  A protein required for transcriptional regulation of Agrobacterium virulence genes spans the cytoplasmic membrane.

Authors:  S C Winans; R A Kerstetter; J E Ward; E W Nester
Journal:  J Bacteriol       Date:  1989-03       Impact factor: 3.490
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  7 in total

1.  Integration of rotation and piston motions in coiled-coil signal transduction.

Authors:  Rong Gao; David G Lynn
Journal:  J Bacteriol       Date:  2007-06-15       Impact factor: 3.490

2.  Localization of the VirA domain involved in acetosyringone-mediated vir gene induction in Agrobacterium tumefaciens.

Authors:  S C Turk; R P van Lange; T J Regensburg-Tuïnk; P J Hooykaas
Journal:  Plant Mol Biol       Date:  1994-08       Impact factor: 4.076

3.  Integration of Agrobacterium tumefaciens T-DNA in the Saccharomyces cerevisiae genome by illegitimate recombination.

Authors:  P Bundock; P J Hooykaas
Journal:  Proc Natl Acad Sci U S A       Date:  1996-12-24       Impact factor: 11.205

4.  Succinoglycan production by Rhizobium meliloti is regulated through the ExoS-ChvI two-component regulatory system.

Authors:  H P Cheng; G C Walker
Journal:  J Bacteriol       Date:  1998-01       Impact factor: 3.490

5.  Glu-255 outside the predicted ChvE binding site in VirA is crucial for sugar enhancement of acetosyringone perception by Agrobacterium tumefaciens.

Authors:  L M Banta; R D Joerger; V R Howitz; A M Campbell; A N Binns
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490

6.  Mutational analysis of the input domain of the VirA protein of Agrobacterium tumefaciens.

Authors:  S L Doty; M C Yu; J I Lundin; J D Heath; E W Nester
Journal:  J Bacteriol       Date:  1996-02       Impact factor: 3.490

7.  Stable and Efficient Agrobacterium-Mediated Genetic Transformation of Larch Using Embryogenic Callus.

Authors:  Yue Song; Xiaoming Bai; Shiwei Dong; Yuning Yang; Hao Dong; Nairui Wang; Hanguo Zhang; Shujuan Li
Journal:  Front Plant Sci       Date:  2020-11-25       Impact factor: 5.753
  7 in total

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