Literature DB >> 8365958

A new method for the concentration of Cryptosporidium oocysts from water.

G Vesey1, J S Slade, M Byrne, K Shepherd, C R Fricker.   

Abstract

A novel method for the concentration of Cryptosporidium oocysts from water has been developed, based upon the precipitation of calcium carbonate. A 10 l water sample is treated by adding solutions of calcium chloride and sodium bicarbonate and raising the pH value to 10 with sodium hydroxide. Crystals of calcium carbonate form and enmesh particles in the Cryptosporidium oocyst size range. The crystals are allowed to settle, the supernatant fluid is discarded and the calcium carbonate precipitate dissolved in sulphamic acid. The sample can be concentrated further by centrifugation. Recoveries of oocysts from seeded samples of deionized, tap and river water were in excess of 68%.

Entities:  

Mesh：

Substances：

Year:  1993        PMID： 8365958     DOI： 10.1111/j.1365-2672.1993.tb03412.x

Source DB:  PubMed          Journal:  J Appl Bacteriol        ISSN： 0021-8847


  25 in total

1.  Occurrence, source, and human infection potential of cryptosporidium and Giardia spp. in source and tap water in shanghai, china.

Authors:  Yaoyu Feng; Xukun Zhao; Jiaxu Chen; Wei Jin; Xiaonong Zhou; Na Li; Lin Wang; Lihua Xiao
Journal:  Appl Environ Microbiol       Date:  2011-04-15       Impact factor: 4.792

Review 2.  Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses.

Authors:  H M Davey; D B Kell
Journal:  Microbiol Rev       Date:  1996-12

3.  Integrated cryptosporidium assay to determine oocyst density, infectivity, and genotype for risk assessment of source and reuse water.

Authors:  Brendon King; Stella Fanok; Renae Phillips; Brooke Swaffer; Paul Monis
Journal:  Appl Environ Microbiol       Date:  2015-03-13       Impact factor: 4.792

4.  Seasonality of Cryptosporidium oocyst detection in surface waters of Meru, Kenya as determined by two isolation methods followed by PCR.

Authors:  John M Muchiri; Luke Ascolillo; Mutuma Mugambi; Titus Mutwiri; Honorine D Ward; Elena N Naumova; Andrey I Egorov; Seth Cohen; James G Else; Jeffrey K Griffiths
Journal:  J Water Health       Date:  2009-03       Impact factor: 1.744

5.  Epidemiology of Cyclospora cayetanensis and other intestinal parasites in a community in Haiti.

Authors:  Adriana S Lopez; Jean M Bendik; Jean Y Alliance; Jacquelin M Roberts; Alexandre J da Silva; Iaci N S Moura; Michael J Arrowood; Mark L Eberhard; Barbara L Herwaldt
Journal:  J Clin Microbiol       Date:  2003-05       Impact factor: 5.948

6.  Sedimentation of free and attached Cryptosporidium oocysts and Giardia cysts in water.

Authors:  G J Medema; F M Schets; P F Teunis; A H Havelaar
Journal:  Appl Environ Microbiol       Date:  1998-11       Impact factor: 4.792

7.  Enumeration and biomass estimation of bacteria in aquifer microcosm studies by flow cytometry.

Authors:  P C Deleo; P Baveye
Journal:  Appl Environ Microbiol       Date:  1996-12       Impact factor: 4.792

Review 8.  Epidemiology and detection as options for control of viral and parasitic foodborne disease.

Authors:  L A Jaykus
Journal:  Emerg Infect Dis       Date:  1997 Oct-Dec       Impact factor: 6.883

9.  Epidemiologic studies of Cyclospora cayetanensis in Guatemala.

Authors:  C Bern; B Hernandez; M B Lopez; M J Arrowood; M A de Mejia; A M de Merida; A W Hightower; L Venczel; B L Herwaldt; R E Klein
Journal:  Emerg Infect Dis       Date:  1999 Nov-Dec       Impact factor: 6.883

10.  Prevalence of Cryptosporidium spp. and Giardia intestinalis in swimming pools, Atlanta, Georgia.

Authors:  Joan M Shields; Elizabeth R Gleim; Michael J Beach
Journal:  Emerg Infect Dis       Date:  2008-06       Impact factor: 6.883
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.