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Literature DB >> 8363565

Identification of the putative hexose-phosphate translocator of amyloplasts from cauliflower buds.

Abstract

Starch synthesis in amyloplasts isolated from cauliflower buds is strongly inhibited by the addition of micromolar concentrations of 4,4'-di-isothiocyano-2,2'-stilbenedisulphonic acid (DIDS). Using [3H]DIDS it was possible to label specifically a 31.6 kDa membrane protein of the envelope fraction of isolated amyloplasts. The intensity of the radioactive label was decreased in the presence of glucose 6-phosphate or dihydroxyacetone phosphate, indicating that this protein might be the amyloplastic hexosephosphate translocator.

Entities: Chemical

Mesh：

Substances：

Year: 1993 PMID： 8363565 PMCID： PMC1134558 DOI： 10.1042/bj2940015

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

9 in total

1. Enzymic capacities of purified cauliflower bud plastids for lipid synthesis and carbohydrate metabolism.

Authors: E P Journet; R Douce
Journal: Plant Physiol Date: 1985-10 Impact factor: 8.340

2. Preparation and characterization of envelope membranes from nongreen plastids.

Authors: C Alban; J Joyard; R Douce
Journal: Plant Physiol Date: 1988-11 Impact factor: 8.340

3. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors: W M Bonner; R A Laskey
Journal: Eur J Biochem Date: 1974-07-01

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

5. Characterization of 4,4'-Diisothiocyano-2,2'-disulfonic Acid Stilbene Inhibition of 3-Phosphoglycerate-Dependent O(2) Evolution in Isolated Chloroplasts : Evidence for a Common Binding Site on the C(4) Phosphate Translocator for 3-Phosphoglycerate, Phosphoenolpyruvate, and Inorganic Phosphate.

Authors: M E Rumpho; G E Edwards
Journal: Plant Physiol Date: 1985-07 Impact factor: 8.340

6. Specific Labeling of the Phosphate Translocator in C(3) and C(4) Mesophyll Chloroplasts by Tritiated Dihydro-DIDS (1,2-Ditritio-1,2-[2,2' -Disulfo-4,4' -Diisothiocyano] Diphenylethane).

Authors: M E Rumpho; G E Edwards; A E Yousif; K Keegstra
Journal: Plant Physiol Date: 1988-04 Impact factor: 8.340

7. Preparation and characterization of membrane fractions enriched in outer and inner envelope membranes from spinach chloroplasts. I. Electrophoretic and immunochemical analyses.

Authors: M A Block; A J Dorne; J Joyard; R Douce
Journal: J Biol Chem Date: 1983-11-10 Impact factor: 5.157

8. Specific labelling of the active site of the phosphate translocator in spinach chloroplasts by 2,4,6-trinitrobenzene sulfonate.

Authors: U I Flügge; H W Heldt
Journal: Biochem Biophys Res Commun Date: 1978-09-14 Impact factor: 3.575

9. Characterization of Glucose-6-Phosphate Incorporation into Starch by Isolated Intact Cauliflower-Bud Plastids.

Authors: H. E. Neuhaus; G. Henrichs; R. Scheibe
Journal: Plant Physiol Date: 1993-02 Impact factor: 8.340

9 in total

5 in total

1. ADP-glucose drives starch synthesis in isolated maize endosperm amyloplasts: characterization of starch synthesis and transport properties across the amyloplast envelope.

Authors: T Möhlmann; J Tjaden; G Henrichs; W P Quick; R Häusler; H E Neuhaus
Journal: Biochem J Date: 1997-06-01 Impact factor: 3.857