| Literature DB >> 8363511 |
C Soiné1, S K Watson, E Rybicki, B Lucio, R M Nordgren, C R Parrish, K A Schat.
Abstract
Chicken infectious anemia virus (CIAV) DNA in infected cell cultures and chicken tissues was detected using a polymerase chain reaction (PCR) assay. The complete CIAV genome of several strains was amplified in two segments with two sets of primer pairs. The DNA segments of four CIAV strains and full-length Cux-1 strain DNA were cloned. After amplification, 100 original genome equivalents were detected by Southern hybridization. The sensitivity of the assay was enhanced considerably by performing a reamplification with nested primers. This modification permitted the detection of one molecule of CIAV DNA. Some problems of the assay and its possible application are discussed.Entities:
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Year: 1993 PMID： 8363511
Source DB: PubMed Journal: Avian Dis ISSN： 0005-2086 Impact factor: 1.577