BACKGROUND: Carbonic anhydrase isoenzymes I and II are present in normal colorectal mucosa. This study aimed to determine if carbonic anhydrase is present in colorectal cancer and what is its potential clinical significance. METHODS: The messenger RNA (mRNA) and protein expression of carbonic anhydrase isoenzyme I were analyzed in fresh specimens of colorectal adenocarcinoma by Northern and Western blots, respectively. The immunohistochemical expression was subsequently studied in a larger number of formalin-fixed surgical specimens. RESULTS: All of 30 normal colon samples had a strong RNA hybridization signal. Only 4 of 39 paired colorectal cancer and none of 9 normal liver samples had detectable levels of carbonic anhydrase mRNA. Isoenzyme I protein expression showed similar results. In a separate group of patients, immunohistochemical studies showed that 16 of 96 colorectal tumors had positive staining cells. All positive tumors were well or moderately differentiated carcinomas (P < 0.05). When analyzed retrospectively, immunoreactive cases were more likely to be in a group with a good outcome (P < 0.01) and to lack vascular invasion (P < 0.01) than negative cases. CONCLUSIONS: The majority of colorectal cancers do not express carbonic anhydrase isoenzyme I. The presence of any isoenzyme I-positive immunoreactive cancer cells may be associated with a more favorable outcome in colorectal cancer.
BACKGROUND: Carbonic anhydrase isoenzymes I and II are present in normal colorectal mucosa. This study aimed to determine if carbonic anhydrase is present in colorectal cancer and what is its potential clinical significance. METHODS: The messenger RNA (mRNA) and protein expression of carbonic anhydrase isoenzyme I were analyzed in fresh specimens of colorectal adenocarcinoma by Northern and Western blots, respectively. The immunohistochemical expression was subsequently studied in a larger number of formalin-fixed surgical specimens. RESULTS: All of 30 normal colon samples had a strong RNA hybridization signal. Only 4 of 39 paired colorectal cancer and none of 9 normal liver samples had detectable levels of carbonic anhydrase mRNA. Isoenzyme I protein expression showed similar results. In a separate group of patients, immunohistochemical studies showed that 16 of 96 colorectal tumors had positive staining cells. All positive tumors were well or moderately differentiated carcinomas (P < 0.05). When analyzed retrospectively, immunoreactive cases were more likely to be in a group with a good outcome (P < 0.01) and to lack vascular invasion (P < 0.01) than negative cases. CONCLUSIONS: The majority of colorectal cancers do not express carbonic anhydrase isoenzyme I. The presence of any isoenzyme I-positive immunoreactive cancer cells may be associated with a more favorable outcome in colorectal cancer.
Authors: A J Kivela; J Saarnio; T J Karttunen; J Kivelä; A K Parkkila; S Pastorekova; J Pastorek; A Waheed; W S Sly; T S Parkkila; H Rajaniemi Journal: Dig Dis Sci Date: 2001-10 Impact factor: 3.199
Authors: Pedro M S Alves; Nicole Lévy; Brian J Stevenson; Hanifa Bouzourene; Grégory Theiler; Gabriel Bricard; Sebastien Viatte; Maha Ayyoub; Henri Vuilleumier; Jean-Claude R Givel; Donata Rimoldi; Daniel E Speiser; C Victor Jongeneel; Pedro J Romero; Frédéric Lévy Journal: Cancer Immun Date: 2008-06-27
Authors: N Miyoshi; H Ishii; K Mimori; Y Takatsuno; H Kim; H Hirose; M Sekimoto; Y Doki; M Mori Journal: Br J Cancer Date: 2009-11-17 Impact factor: 7.640