Cyclic stretch increases alkaline phosphatase activity of osteoblast-like cells: a role for prostaglandin E2.

We investigated the effects of applied cyclic stretch on osteoblast-like cells using a computerized, pressure-operated instrument that physically deforms the cells. Cyclic stretch increased DNA and collagenous protein synthesis and enhanced alkaline phosphatase activity in the cells. These effects were considered to be regulated through prostaglandin E2 production since this stretch reduced the levels of prostaglandin E2 in the medium and when prostaglandin E2 was added exogenously, there was a significant decrease in the alkaline phosphatase activity.