Literature DB >> 8358230

Action of rat liver Gal beta 1-4GlcNAc alpha(2-6)-sialyltransferase on Man beta 1-4GlcNAc beta-OMe, GalNAc beta 1-4GlcNAc beta-OMe, Glc beta 1-4GlcNAc beta-OMe and GlcNAc beta 1-4GlcNAc beta-OMe as synthetic substrates.

C H Hokke1, J G van der Ven, J P Kamerling, J F Vliegenthart.   

Abstract

Incubation of synthetic Man beta 1-4GlcNAc beta-OMe, GalNAc beta 1-4GlcNAc beta-OMe, Glc beta 1-4GlcNAc beta-OMe, and GlcNAc beta 1-4GlcNAc beta-OMe with CMP-Neu5Ac and rat liver Gal beta 1-4GlcNAc alpha(2-6)-sialyltransferase resulted in the formation of Neu5Ac alpha 2-6Man beta 1-4GlcNAc beta-OMe, Neu5Ac alpha 2-6GalNAc beta 1-4GlcNAc beta-OMe, Neu5Ac alpha 2-6Glc beta 1-4GlcNAc beta-OMe and Neu5Ac alpha 2-6GlcNAc beta 1-4GlcNAc beta-OMe, respectively. Under conditions which led to quantitative conversion of Gal beta 1-4GlcNAc beta-OEt into Neu5Ac alpha 2-6Gal beta 1-4GlcNAc beta-OEt, the aforementioned products were obtained in yields of 4%, 48%, 16% and 8%, respectively. HPLC on Partisil 10 SAX was used to isolate the various sialyltrisaccharides, and identification was carried out using 1- and 2-dimensional 500-MHz 1H-NMR spectroscopy.

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Year:  1993        PMID： 8358230     DOI： 10.1007/bf00731191

Source DB:  PubMed          Journal:  Glycoconj J        ISSN： 0282-0080            Impact factor:   2.916


  16 in total

Review 1.  Glycoprotein sialyltransferases in eucaryotic cells.

Authors:  P Broquet; H Baubichon-Cortay; P George; P Louisot
Journal:  Int J Biochem       Date:  1991

Review 2.  Characterization of sialic acids.

Authors:  R Schauer
Journal:  Methods Enzymol       Date:  1978       Impact factor: 1.600

3.  The asparagine-linked oligosaccharides on bovine fetuin. Structural analysis of N-glycanase-released oligosaccharides by 500-megahertz 1H NMR spectroscopy.

Authors:  E D Green; G Adelt; J U Baenziger; S Wilson; H Van Halbeek
Journal:  J Biol Chem       Date:  1988-12-05       Impact factor: 5.157

4.  Protein--sugar interactions. A nuclear magnetic resonance investigation of the binding of O-methyl-di-N-acetyl-beta-chitobioside to wheat germ agglutinin (lectin).

Authors:  J P Grivet; F Delmotte; M Monsigny
Journal:  FEBS Lett       Date:  1978-04-15       Impact factor: 4.124

5.  Sialyl-alpha 2-6-mannosyl-beta 1-4-N-acetylglucosamine, a novel compound occurring in urine of patients with beta-mannosidosis.

Authors:  J van Pelt; L Dorland; M Duran; C H Hokke; J P Kamerling; J F Vliegenthart
Journal:  J Biol Chem       Date:  1990-11-15       Impact factor: 5.157

6.  Structures of asparagine-linked oligosaccharides of the glycoprotein fetuin having sialic acid linked to N-acetylglucosamine.

Authors:  D A Cumming; C G Hellerqvist; M Harris-Brandts; S W Michnick; J P Carver; B Bendiak
Journal:  Biochemistry       Date:  1989-07-25       Impact factor: 3.162

7.  Conformation of the complex oligosaccharides of glycoproteins. A vacuum ultraviolet circular dichroism study.

Authors:  C A Bush; V K Dua; S Ralapati; C D Warren; G Spik; G Strecker; J Montreuil
Journal:  J Biol Chem       Date:  1982-07-25       Impact factor: 5.157

8.  Sialylation of glycoprotein oligosaccharides N-linked to asparagine. Enzymatic characterization of a Gal beta 1 to 3(4)GlcNAc alpha 2 to 3 sialyltransferase and a Gal beta 1 to 4GlcNAc alpha 2 to 6 sialyltransferase from rat liver.

Authors:  J Weinstein; U de Souza-e-Silva; J C Paulson
Journal:  J Biol Chem       Date:  1982-11-25       Impact factor: 5.157

9.  The Asn-linked carbohydrate chains of human Tamm-Horsfall glycoprotein of one male. Novel sulfated and novel N-acetylgalactosamine-containing N-linked carbohydrate chains.

Authors:  K Hård; G Van Zadelhoff; P Moonen; J P Kamerling; F G Vliegenthart
Journal:  Eur J Biochem       Date:  1992-11-01

10.  A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.

Authors:  A L Chan; H R Morris; M Panico; A T Etienne; M E Rogers; P Gaffney; L Creighton-Kempsford; A Dell
Journal:  Glycobiology       Date:  1991-03       Impact factor: 4.313
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  2 in total

Review 1.  Genetic engineering of recombinant glycoproteins and the glycosylation pathway in mammalian host cells.

Authors:  E Grabenhorst; P Schlenke; S Pohl; M Nimtz; H S Conradt
Journal:  Glycoconj J       Date:  1999-02       Impact factor: 2.916

2.  Construction and characterization of stably transfected BHK-21 cells with human-type sialylation characteristic.

Authors:  P Schlenke; E Grabenhorst; M Nimtz; H S Conradt
Journal:  Cytotechnology       Date:  1999-07       Impact factor: 2.058
  2 in total

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