Literature DB >> 8356117

Clostridium perfringens-Escherichia coli shuttle vectors that carry single antibiotic resistance determinants.

T L Bannam1, J I Rood.   

Abstract

Two versatile Clostridium perfringens-Escherichia coli shuttle vectors were constructed. Each plasmid carried a single antibiotic resistance gene which was expressed in both organisms. The plasmid pJIR750 encoded resistance to chloramphenicol and pJIR751 encoded resistance to erythromycin. Each plasmid contained the pUC18-derived multiple cloning site and the lacZ' gene which enabled direct screening for recombinants in E. coli. These plasmids should prove invaluable for the genetic manipulation of C. perfringens.

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Year:  1993        PMID： 8356117     DOI： 10.1006/plas.1993.1025

Source DB:  PubMed          Journal:  Plasmid        ISSN： 0147-619X            Impact factor:   3.466


  67 in total

1.  The VirSR two-component signal transduction system regulates NetB toxin production in Clostridium perfringens.

Authors:  Jackie K Cheung; Anthony L Keyburn; Glen P Carter; Anouk L Lanckriet; Filip Van Immerseel; Robert J Moore; Julian I Rood
Journal:  Infect Immun       Date:  2010-05-10       Impact factor: 3.441

2.  The SKHR motif is required for biological function of the VirR response regulator from Clostridium perfringens.

Authors:  Sheena McGowan; Jennifer R O'Connor; Jackie K Cheung; Julian I Rood
Journal:  J Bacteriol       Date:  2003-10       Impact factor: 3.490

3.  Expression of a cloned cyclopropane fatty acid synthase gene reduces solvent formation in Clostridium acetobutylicum ATCC 824.

Authors:  Yinsuo Zhao; Lucia A Hindorff; Amy Chuang; Melanie Monroe-Augustus; Michael Lyristis; Mary L Harrison; Frederick B Rudolph; George N Bennett
Journal:  Appl Environ Microbiol       Date:  2003-05       Impact factor: 4.792

4.  Random mutagenesis of Clostridium cellulolyticum by using a Tn1545 derivative.

Authors:  Jean-Charles Blouzard; Odile Valette; Chantal Tardif; Pascale de Philip
Journal:  Appl Environ Microbiol       Date:  2010-04-30       Impact factor: 4.792

5.  Evaluating the involvement of alternative sigma factors SigF and SigG in Clostridium perfringens sporulation and enterotoxin synthesis.

Authors:  Jihong Li; Bruce A McClane
Journal:  Infect Immun       Date:  2010-07-19       Impact factor: 3.441

6.  Utility of the clostridial site-specific recombinase TnpX to clone toxic-product-encoding genes and selectively remove genomic DNA fragments.

Authors:  Vicki Adams; Radhika Bantwal; Lauren Stevenson; Jackie K Cheung; Milena M Awad; Joel Nicholson; Glen P Carter; Kate E Mackin; Julian I Rood; Dena Lyras
Journal:  Appl Environ Microbiol       Date:  2014-06       Impact factor: 4.792

7.  Efficient gene transfer and targeted mutagenesis in Fusobacterium nucleatum.

Authors:  Susan Kinder Haake; Sean Yoder; Sharon Hunt Gerardo
Journal:  Plasmid       Date:  2005-08-22       Impact factor: 3.466

8.  Carbon catabolite repression of type IV pilus-dependent gliding motility in the anaerobic pathogen Clostridium perfringens.

Authors:  Marcelo Mendez; I-Hsiu Huang; Kaori Ohtani; Roberto Grau; Tohru Shimizu; Mahfuzur R Sarker
Journal:  J Bacteriol       Date:  2007-11-02       Impact factor: 3.490

9.  Expression from the Clostridium perfringens cpe promoter in C. perfringens and Bacillus subtilis.

Authors:  S B Melville; R Labbe; A L Sonenshein
Journal:  Infect Immun       Date:  1994-12       Impact factor: 3.441

10.  Improvement of butanol production in Clostridium acetobutylicum through enhancement of NAD(P)H availability.

Authors:  Feng Qi; Chandresh Thakker; Fayin Zhu; Matthew Pena; Ka-Yiu San; George N Bennett
Journal:  J Ind Microbiol Biotechnol       Date:  2018-08-23       Impact factor: 3.346
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