Literature DB >> 8356082

Serine/threonine phosphorylation regulates binding of C hnRNP proteins to pre-mRNA.

S H Mayrand1, P Dwen, T Pederson.   

Abstract

The C hnRNP proteins bind to nascent pre-mRNA and are thought to participate in an early step of the pre-mRNA splicing pathway. We report here that C hnRNP proteins are phosphorylated by a casein kinase II activity in a HeLa cell nuclear extract and that dephosphorylation of C hnRNP proteins is inhibited by the specific protein-serine/threonine-phosphatase 1/2A inhibitor okadaic acid. We further find that dephosphorylation of C hnRNP proteins is required for their binding to adenovirus and human beta-globin pre-mRNAs. These results indicate that the participation of C hnRNP proteins in pre-spliceosome assembly is coupled to a dynamic cycle of their phosphorylation and dephosphorylation.

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Year:  1993        PMID： 8356082      PMCID： PMC47223          DOI： 10.1073/pnas.90.16.7764

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  53 in total

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Journal:  Biochim Biophys Acta       Date:  1977-06-10

2.  Ser/Thr-specific protein phosphatases are required for both catalytic steps of pre-mRNA splicing.

Authors:  J E Mermoud; P Cohen; A I Lamond
Journal:  Nucleic Acids Res       Date:  1992-10-25       Impact factor: 16.971

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Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

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Authors:  T Pederson
Journal:  J Mol Biol       Date:  1974-02-25       Impact factor: 5.469

5.  Thiophosphorylation of U1-70K protein inhibits pre-mRNA splicing.

Authors:  J Tazi; U Kornstädt; F Rossi; P Jeanteur; G Cathala; C Brunel; R Lührmann
Journal:  Nature       Date:  1993-05-20       Impact factor: 49.962

6.  Characterization of heterogeneous nuclear RNA-protein complexes in vivo with monoclonal antibodies.

Authors:  G Dreyfuss; Y D Choi; S A Adam
Journal:  Mol Cell Biol       Date:  1984-06       Impact factor: 4.272

7.  Normal and mutant human beta-globin pre-mRNAs are faithfully and efficiently spliced in vitro.

Authors:  A R Krainer; T Maniatis; B Ruskin; M R Green
Journal:  Cell       Date:  1984-04       Impact factor: 41.582

8.  Adenosine phosphorothioates (ATP alpha S and ATP tau S) differentially affect the two steps of mammalian pre-mRNA splicing.

Authors:  J Tazi; M C Daugeron; G Cathala; C Brunel; P Jeanteur
Journal:  J Biol Chem       Date:  1992-03-05       Impact factor: 5.157

9.  Casein kinase II is a negative regulator of c-Jun DNA binding and AP-1 activity.

Authors:  A Lin; J Frost; T Deng; T Smeal; N al-Alawi; U Kikkawa; T Hunter; D Brenner; M Karin
Journal:  Cell       Date:  1992-09-04       Impact factor: 41.582

10.  Messenger RNA processing and nuclear structure: isolation of nuclear ribonucleoprotein particles containing beta-globin messenger RNA precursors.

Authors:  T Pederson; N G Davis
Journal:  J Cell Biol       Date:  1980-10       Impact factor: 10.539
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  28 in total

1.  Differentiation-induced internal translation of c-sis mRNA: analysis of the cis elements and their differentiation-linked binding to the hnRNP C protein.

Authors:  O Sella; G Gerlitz; S Y Le; O Elroy-Stein
Journal:  Mol Cell Biol       Date:  1999-08       Impact factor: 4.272

2.  Recruitment of a basal polyadenylation factor by the upstream sequence element of the human lamin B2 polyadenylation signal.

Authors:  S Brackenridge; N J Proudfoot
Journal:  Mol Cell Biol       Date:  2000-04       Impact factor: 4.272

3.  p53 targets identified by protein expression profiling.

Authors:  Rubaiyat Rahman-Roblick; Uwe Johannes Roblick; Ulf Hellman; Paolo Conrotto; Tao Liu; Susanne Becker; Daniel Hirschberg; Hans Jörnvall; Gert Auer; Klas G Wiman
Journal:  Proc Natl Acad Sci U S A       Date:  2007-03-19       Impact factor: 11.205

Review 4.  Adipokines and the blood-brain barrier.

Authors:  Weihong Pan; Abba J Kastin
Journal:  Peptides       Date:  2007-05-06       Impact factor: 3.750

5.  Contribution of the individual subunits of protein kinase CK2 and of hPrp3p to the splicing process.

Authors:  Janka Dörr; Sabine Kartarius; Claudia Götz; Mathias Montenarh
Journal:  Mol Cell Biochem       Date:  2008-06-14       Impact factor: 3.396

6.  Tomato SlSnRK1 protein interacts with and phosphorylates βC1, a pathogenesis protein encoded by a geminivirus β-satellite.

Authors:  Qingtang Shen; Zhou Liu; Fengming Song; Qi Xie; Linda Hanley-Bowdoin; Xueping Zhou
Journal:  Plant Physiol       Date:  2011-09-01       Impact factor: 8.340

7.  Serine/threonine phosphatase 1 modulates the subnuclear distribution of pre-mRNA splicing factors.

Authors:  T Misteli; D L Spector
Journal:  Mol Biol Cell       Date:  1996-10       Impact factor: 4.138

8.  A discrete 3' region of U6 small nuclear RNA modulates the phosphorylation cycle of the C1 heterogeneous nuclear ribonucleoprotein particle protein.

Authors:  S H Mayrand; P A Fung; T Pederson
Journal:  Mol Cell Biol       Date:  1996-03       Impact factor: 4.272

9.  Coupling of signal transduction to alternative pre-mRNA splicing by a composite splice regulator.

Authors:  H König; H Ponta; P Herrlich
Journal:  EMBO J       Date:  1998-05-15       Impact factor: 11.598

10.  Chloroplast endoribonuclease p54 involved in RNA 3'-end processing is regulated by phosphorylation and redox state.

Authors:  K Liere; G Link
Journal:  Nucleic Acids Res       Date:  1997-06-15       Impact factor: 16.971
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