Literature DB >> 8355424

An improved method for perforated patch recordings using nystatin-fluorescein mixture.

H Yawo1, N Chuhma.   

Abstract

This paper describes an improved method for solubilizing nystatin in an aqueous solution without the aid of organic solvents. The patch pipette was filled with a solution of nystatin and fluorescein sodium mixed in a molar ratio of 1:10. The success rate of perforated patch recordings was substantially improved, and the access resistance of 20-40 M omega was readily achieved.

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Year:  1993        PMID： 8355424     DOI： 10.2170/jjphysiol.43.267

Source DB:  PubMed          Journal:  Jpn J Physiol        ISSN： 0021-521X


  5 in total

1.  Alterations in basal and glucose-stimulated voltage-dependent Ca2+ channel activities in pancreatic beta cells of non-insulin-dependent diabetes mellitus GK rats.

Authors:  S Kato; H Ishida; Y Tsuura; K Tsuji; M Nishimura; M Horie; T Taminato; S Ikehara; H Odaka; I Ikeda; Y Okada; Y Seino
Journal:  J Clin Invest       Date:  1996-06-01       Impact factor: 14.808

2.  Electrophysiological analysis of the negative chronotropic effect of endothelin-1 in rabbit sinoatrial node cells.

Authors:  K Ono; H Masumiya; A Sakamoto; G Christé; T Shijuku; H Tanaka; K Shigenobu; Y Ozaki
Journal:  J Physiol       Date:  2001-12-01       Impact factor: 5.182

3.  mu-Opioid receptor inhibits N-type Ca2+ channels in the calyx presynaptic terminal of the embryonic chick ciliary ganglion.

Authors:  K Endo; H Yawo
Journal:  J Physiol       Date:  2000-05-01       Impact factor: 5.182

4.  Intrinsic plasticity induced by group II metabotropic glutamate receptors via enhancement of high-threshold KV currents in sound localizing neurons.

Authors:  W R Hamlet; Y Lu
Journal:  Neuroscience       Date:  2016-03-08       Impact factor: 3.590

5.  Use of artificial ion channels for quasi-intracellular recording of cerebral cortex neuron activity.

Authors:  D N Lenkov
Journal:  Neurosci Behav Physiol       Date:  1997 Nov-Dec
  5 in total

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