Literature DB >> 8352799

Molecular cloning of polymorphic markers on RLGS gel using the spot target cloning method.

S Hirotsune1, H Shibata, Y Okazaki, H Sugino, H Imoto, N Sasaki, K Hirose, H Okuizumi, M Muramatsu, C Plass.   

Abstract

A new method for target cloning of DNA fragments corresponding to spots on the two-dimensional restriction landmark genomic scanning (RLGS) profile has been developed (targeted spot cloning). We used a Not I restriction trapper to select target DNA fragments from Not I, Eco RV double digests of genomic DNA. The use of the restriction trapper substantially reduces the background clones that are established from the direct recovery of RLGS spot DNA from the two-dimensional gels. Genomic DNA clones were isolated in this study as mouse genome markers for 58 spot loci that were previously characterized using RLGS spot mapping. This method provides a powerful tool for isolating DNA clones after their identification by RLGS system.

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Year:  1993        PMID： 8352799     DOI： 10.1006/bbrc.1993.1981

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  9 in total

1.  Virtual genome scan: a tool for restriction landmark-based scanning of the human genome.

Authors:  J M Rouillard; A E Erson; R Kuick; J Asakawa ; K Wimmer; M Muleris; E M Petty; S Hanash
Journal:  Genome Res       Date:  2001-08       Impact factor: 9.043

2.  Application of the restriction landmark genomic scanning (RLGS) method to rice cultivars as a new fingerprinting technique.

Authors:  M Kawase
Journal:  Theor Appl Genet       Date:  1994-12       Impact factor: 5.699

3.  Increase of methicillin resistance in Staphylococcus aureus caused by deletion of a gene whose product is homologous to lytic enzymes.

Authors:  T Fujimura; K Murakami
Journal:  J Bacteriol       Date:  1997-10       Impact factor: 3.490

4.  Restriction landmark cDNA scanning (RLCS): a novel cDNA display system using two-dimensional gel electrophoresis.

Authors:  H Suzuki; T Yaoi; J Kawai; A Hara; G Kuwajima; S Wantanabe
Journal:  Nucleic Acids Res       Date:  1996-01-15       Impact factor: 16.971

5.  A unique downregulation of h2-calponin gene expression in Down syndrome: a possible attenuation mechanism for fetal survival by methylation at the CpG island in the trisomic chromosome 21.

Authors:  J Kuromitsu; H Yamashita; H Kataoka; T Takahara; M Muramatsu; T Sekine; N Okamoto; Y Furuichi; Y Hayashizaki
Journal:  Mol Cell Biol       Date:  1997-02       Impact factor: 4.272

6.  Direct detection and isolation of restriction landmark genomic scanning (RLGS) spot DNA markers tightly linked to a specific trait by using the RLGS spot-bombing method.

Authors:  Y Okazaki; K Hirose; S Hirotsune; H Okuizumi; N Sasaki; T Ohsumi; A Yoshiki; M Kusakabe; M Muramatsu; J Kawai
Journal:  Proc Natl Acad Sci U S A       Date:  1995-06-06       Impact factor: 11.205

7.  Methylation profiles of genomic DNA of mouse developmental brain detected by restriction landmark genomic scanning (RLGS) method.

Authors:  J Kawai; S Hirotsune; K Hirose; S Fushiki; S Watanabe; Y Hayashizaki
Journal:  Nucleic Acids Res       Date:  1993-12-11       Impact factor: 16.971

8.  Comparison of DNA methylation patterns among mouse cell lines by restriction landmark genomic scanning.

Authors:  J Kawai; K Hirose; S Fushiki; S Hirotsune; N Ozawa; A Hara; Y Hayashizaki; S Watanabe
Journal:  Mol Cell Biol       Date:  1994-11       Impact factor: 4.272

9.  A genetic linkage map of the mouse using restriction landmark genomic scanning (RLGS).

Authors:  Y Hayashizaki; S Hirotsune; Y Okazaki; H Shibata; A Akasako; M Muramatsu; J Kawai; T Hirasawa; S Watanabe; T Shiroishi
Journal:  Genetics       Date:  1994-12       Impact factor: 4.562
  9 in total

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