Literature DB >> 8352795

Cloning and expression in Escherichia coli of Thermotoga neapolitana genes coding for enzymes of carbohydrate substrate degradation.

O N Dakhova1, N E Kurepina, V V Zverlov, V A Svetlichnyi, G A Velikodvorskaya.   

Abstract

A genomic library of thermophilic anaerobic eubacterium Thermatoga neapolitana was constructed in E. coli using the pTZ19R plasmid vector. Some groups of recombinant clones with different cellulase activities were revealed: clones carrying genes for an 1,4-beta-glucanases, 1,3-beta-glucanases, beta-xylanases, beta-glucosidases and beta-xylosidases. One clone possessing avicelase activity was obtained. Some clones were selected with amylolytic activities toward amylose, amylopectin and pullulan.

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Year:  1993        PMID： 8352795     DOI： 10.1006/bbrc.1993.1974

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  11 in total

1.  An endoglucanase, EglA, from the hyperthermophilic archaeon Pyrococcus furiosus hydrolyzes beta-1,4 bonds in mixed-linkage (1-->3),(1-->4)-beta-D-glucans and cellulose.

Authors:  M W Bauer; L E Driskill; W Callen; M A Snead; E J Mathur; R M Kelly
Journal:  J Bacteriol       Date:  1999-01       Impact factor: 3.490

2.  The multidomain xylanase A of the hyperthermophilic bacterium Thermotoga neapolitana is extremely thermoresistant.

Authors:  V Zverlov; K Piotukh; O Dakhova; G Velikodvorskaya; R Borriss
Journal:  Appl Microbiol Biotechnol       Date:  1996-03       Impact factor: 4.813

3.  Cloning and characterization of the glucooligosaccharide catabolic pathway beta-glucan glucohydrolase and cellobiose phosphorylase in the marine hyperthermophile Thermotoga neapolitana.

Authors:  D A Yernool; J K McCarthy; D E Eveleigh; J D Bok
Journal:  J Bacteriol       Date:  2000-09       Impact factor: 3.490

4.  Purification and characterization of extremely thermostable beta-mannanase, beta-mannosidase, and alpha-galactosidase from the hyperthermophilic eubacterium Thermotoga neapolitana 5068.

Authors:  G D Duffaud; C M McCutchen; P Leduc; K N Parker; R M Kelly
Journal:  Appl Environ Microbiol       Date:  1997-01       Impact factor: 4.792

5.  Cloning and sequence analysis of a new cellulase gene encoding CelK, a major cellulosome component of Clostridium thermocellum: evidence for gene duplication and recombination.

Authors:  I Kataeva; X L Li; H Chen; S K Choi; L G Ljungdahl
Journal:  J Bacteriol       Date:  1999-09       Impact factor: 3.490

6.  An expression-driven approach to the prediction of carbohydrate transport and utilization regulons in the hyperthermophilic bacterium Thermotoga maritima.

Authors:  Shannon B Conners; Clemente I Montero; Donald A Comfort; Keith R Shockley; Matthew R Johnson; Swapnil R Chhabra; Robert M Kelly
Journal:  J Bacteriol       Date:  2005-11       Impact factor: 3.490

7.  Transcriptional analysis of biofilm formation processes in the anaerobic, hyperthermophilic bacterium Thermotoga maritima.

Authors:  Marybeth A Pysz; Shannon B Conners; Clemente I Montero; Keith R Shockley; Matthew R Johnson; Donald E Ward; Robert M Kelly
Journal:  Appl Environ Microbiol       Date:  2004-10       Impact factor: 4.792

8.  Purification of Thermotoga maritima enzymes for the degradation of cellulosic materials.

Authors:  K Bronnenmeier; A Kern; W Liebl; W L Staudenbauer
Journal:  Appl Environ Microbiol       Date:  1995-04       Impact factor: 4.792

9.  Three glycoside hydrolase family 12 enzymes display diversity in substrate specificities and synergistic action between each other.

Authors:  Zhu Zhu; Jingyao Qu; Lele Yu; Xukai Jiang; Guodong Liu; Lushan Wang; Yinbo Qu; Yuqi Qin
Journal:  Mol Biol Rep       Date:  2019-07-29       Impact factor: 2.316

10.  Loss of a conserved salt bridge in bacterial glycosyl hydrolase BgIM-G1 improves substrate binding in temperate environments.

Authors:  Dipali Mhaindarkar; Raphael Gasper; Natalie Lupilov; Eckhard Hofmann; Lars I Leichert
Journal:  Commun Biol       Date:  2018-10-17
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