Literature DB >> 8351198

Contractile protein gene expression in serum-free cultured adult rat cardiac myocytes.

I Dubus1, L Rappaport, A Barrieux, A M Lompré, K Schwartz, J L Samuel.   

Abstract

The effects of two adhesion substrates (serum and laminin) and time in culture on the expression of genes encoding myosin heavy chain (MHC) isoforms and alpha-skeletal actin were analysed in myocytes isolated from adult rat heart and maintained in serum-free culture. Relative messenger ribonucleic acid (mRNA) abundances were quantitated by dot-blot analysis. Gene expression was not influenced by the substrate used. Time in culture induced a decrease in total mRNA abundance and an up-regulation of beta-MHC and alpha-skeletal actin genes. It is proposed that atrophy of adult myocytes is associated with a pattern of gene expression similar to the fetal program.

Entities:  

Mesh：

Substances：

Year:  1993        PMID： 8351198     DOI： 10.1007/bf00374941

Source DB:  PubMed          Journal:  Pflugers Arch        ISSN： 0031-6768            Impact factor:   3.657


  23 in total

1.  Contractile activity modulates myosin heavy chain-beta expression in neonatal rat heart cells.

Authors:  A M Samarel; G L Engelmann
Journal:  Am J Physiol       Date:  1991-10

2.  Effect of thyroid hormone on the accumulation of mRNA for skeletal and cardiac alpha-actin in hearts from normal and hypophysectomized rats.

Authors:  S Winegrad; C Wisnewsky; K Schwartz
Journal:  Proc Natl Acad Sci U S A       Date:  1990-04       Impact factor: 11.205

3.  Synthesis of stress proteins in rat cardiac myocytes 2-4 days after imposition of hemodynamic overload.

Authors:  C Delcayre; J L Samuel; F Marotte; M Best-Belpomme; J J Mercadier; L Rappaport
Journal:  J Clin Invest       Date:  1988-08       Impact factor: 14.808

4.  Hybridization of oligo(dT) to RNA on nitrocellulose.

Authors:  C B Harley
Journal:  Gene Anal Tech       Date:  1987 Mar-Apr

5.  Differentiation of adult rat cardiac myocytes in cell culture.

Authors:  L B Bugaisky; R Zak
Journal:  Circ Res       Date:  1989-03       Impact factor: 17.367

6.  Stimulation of hypertrophy of cultured neonatal rat heart cells through an alpha 1-adrenergic receptor and induction of beating through an alpha 1- and beta 1-adrenergic receptor interaction. Evidence for independent regulation of growth and beating.

Authors:  P Simpson
Journal:  Circ Res       Date:  1985-06       Impact factor: 17.367

7.  Genetic analysis of the interaction between cardiac and skeletal actin gene expression in striated muscle of the mouse.

Authors:  S Alonso; I Garner; J Vandekerckhove; M Buckingham
Journal:  J Mol Biol       Date:  1990-02-20       Impact factor: 5.469

8.  Nucleotide sequence of the rat skeletal muscle actin gene.

Authors:  R Zakut; M Shani; D Givol; S Neuman; D Yaffe; U Nudel
Journal:  Nature       Date:  1982-08-26       Impact factor: 49.962

9.  Nonsynchronous accumulation of alpha-skeletal actin and beta-myosin heavy chain mRNAs during early stages of pressure-overload--induced cardiac hypertrophy demonstrated by in situ hybridization.

Authors:  S Schiaffino; J L Samuel; D Sassoon; A M Lompré; I Garner; F Marotte; M Buckingham; L Rappaport; K Schwartz
Journal:  Circ Res       Date:  1989-05       Impact factor: 17.367

10.  Atrial natriuretic factor gene expression in rat ventricle during experimental hypertension.

Authors:  J J Mercadier; J L Samuel; J B Michel; M A Zongazo; D de la Bastie; A M Lompre; C Wisnewsky; L Rappaport; B Levy; K Schwartz
Journal:  Am J Physiol       Date:  1989-09
View more
  3 in total

1.  Characterization of a hyperpolarization-activated current in dedifferentiated adult rat ventricular cells in primary culture.

Authors:  N Farès; P Bois; J Lenfant; D Potreau
Journal:  J Physiol       Date:  1998-01-01       Impact factor: 5.182

2.  Transformation of adult ventricular myocytes with the temperature sensitive A58 (tsA58) mutant of the SV40 large T antigen.

Authors:  C Miller; J Rulfs; S R Jaspers; M Buckholt; T B Miller
Journal:  Mol Cell Biochem       Date:  1994-07-13       Impact factor: 3.396

3.  Endogenous endothelin 1 mediates angiotensin II-induced hypertrophy in electrically paced cardiac myocytes through EGFR transactivation, reactive oxygen species and NHE-1.

Authors:  María V Correa; Mariela B Nolly; Claudia I Caldiz; Gladys E Chiappe de Cingolani; Horacio E Cingolani; Irene L Ennis
Journal:  Pflugers Arch       Date:  2013-12-11       Impact factor: 3.657
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.