Literature DB >> 8350869

Role of protein kinase C in oxidant--mediated activation of phospholipase A2 in rabbit pulmonary arterial smooth muscle cells.

S Chakraborti1, J R Michael.   

Abstract

The present study was undertaken to test the hypothesis that activation of cell membrane associated protein kinase C (PKC) plays a role in stimulating cell membrane associated phospholipase A2 (PLA2) activity, and subsequent liberation of arachidonic acid (AA) under exposure of rabbit pulmonary arterial smooth muscle cells to the oxidant hydrogen peroxide (H2O2). Exposure of the smooth muscle cells to H2O2 dose-dependently stimulates [14C] AA release, and enhances the cell membrane associated PLA2 activity. Pretreatment of the cells with protein kinase C (PKC) inhibitors H7 and sphingosine prevent the cell membrane associated PLA2 activity, and AA release caused by H2O2. Treatment of the smooth muscle cells with H2O2 stimulates the cell membrane associated PKC activity. Pretreatment of the cells with an antioxidant vitamin E prevents H2O2 caused stimulation of the cell membrane associated PKC activity. The cell membrane associated PLA2 and PKC activities correlate linearly. These results suggest that H2O2 caused stimulation of the smooth muscle cell membrane associated PLA2 activity, and subsequent liberation of AA can occur through an increase in the activity of the cell membrane associated PKC.

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Year:  1993        PMID： 8350869     DOI： 10.1007/bf00925732

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  19 in total

1.  Distribution of membrane marker enzymes in cultured arterial endothelial and smooth muscle cells. The subcellular location of oleoyl-CoA:1-acyl-sn-glycero-3-phosphocholine acyltransferase.

Authors:  W W Magargal; E S Dickinson; L L Slakey
Journal:  J Biol Chem       Date:  1978-11-25       Impact factor: 5.157

2.  Pharmacomechanical coupling in smooth muscle may involve phosphatidylinositol metabolism.

Authors:  C B Baron; M Cunningham; J F Strauss; R F Coburn
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

3.  Enhancement of arachidonic acid liberation by protein kinase C activator is partially dependent on extracellular Na+ in rabbit platelets.

Authors:  S Akiba; T Sato; T Fujii
Journal:  FEBS Lett       Date:  1989-08-28       Impact factor: 4.124

Review 4.  Studies and perspectives of protein kinase C.

Authors:  Y Nishizuka
Journal:  Science       Date:  1986-07-18       Impact factor: 47.728

Review 5.  Na+/H+ exchange and Ca2+ influx.

Authors:  W Siffert; J W Akkerman
Journal:  FEBS Lett       Date:  1989-12-18       Impact factor: 4.124

6.  Oxygen-radical-mediated permeability edema and vasoconstriction in isolated perfused rabbit lungs.

Authors:  R M Tate; K M Vanbenthuysen; D M Shasby; I F McMurtry; J E Repine
Journal:  Am Rev Respir Dis       Date:  1982-11

7.  Platelet activation--a role for a 40K anti-phospholipase A2 protein indistinguishable from lipocortin.

Authors:  L Touqui; B Rothhut; A M Shaw; A Fradin; B B Vargaftig; F Russo-Marie
Journal:  Nature       Date:  1986 May 8-14       Impact factor: 49.962

Review 8.  Mechanisms of action of calcium-mobilizing agonists: some variations on a young theme.

Authors:  J H Exton
Journal:  FASEB J       Date:  1988-08       Impact factor: 5.191

9.  Vitamin E inhibits protein kinase C activity.

Authors:  C W Mahoney; A Azzi
Journal:  Biochem Biophys Res Commun       Date:  1988-07-29       Impact factor: 3.575

10.  Role of serine esterase in hydrogen peroxide-mediated activation of phospholipase A2 in rabbit pulmonary arterial smooth muscle cells.

Authors:  S Chakraborti; S K Batabyal; G Dutta; J R Michael
Journal:  Indian J Biochem Biophys       Date:  1992-12       Impact factor: 1.918
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  10 in total

1.  Cross-talk between NADPH oxidase-PKCα-p(38)MAPK and NF-κB-MT1MMP in activating proMMP-2 by ET-1 in pulmonary artery smooth muscle cells.

Authors:  Jaganmay Sarkar; Animesh Chowdhury; Tapati Chakraborti; Sajal Chakraborti
Journal:  Mol Cell Biochem       Date:  2016-02-24       Impact factor: 3.396

2.  Role of membrane associated serine esterase in the activation of phospholipase A2 by calcium ionophore (A23187) in pulmonary arterial smooth muscle cells.

Authors:  S Chakraborti; S K Batabyal; J R Michael; T Sanyal
Journal:  Mol Cell Biochem       Date:  1994-01-26       Impact factor: 3.396

3.  Hydrogen peroxide induces a greater contraction in mesenteric arteries of spontaneously hypertensive rats through thromboxane A(2) production.

Authors:  Y J Gao; R M Lee
Journal:  Br J Pharmacol       Date:  2001-12       Impact factor: 8.739

4.  Profiling the role of mammalian target of rapamycin in the vascular smooth muscle metabolome in pulmonary arterial hypertension.

Authors:  Tatiana V Kudryashova; Dmitry A Goncharov; Andressa Pena; Kaori Ihida-Stansbury; Horace DeLisser; Steven M Kawut; Elena A Goncharova
Journal:  Pulm Circ       Date:  2015-12       Impact factor: 3.017

Review 5.  Antioxidant therapy against cerebral vasospasm following aneurysmal subarachnoid hemorrhage.

Authors:  T Asano; T Matsui
Journal:  Cell Mol Neurobiol       Date:  1999-02       Impact factor: 5.046

6.  Role of MMP-2 in PKCdelta-mediated inhibition of Na+ dependent Ca2+ uptake in microsomes of pulmonary smooth muscle: involvement of a pertussis toxin sensitive protein.

Authors:  Sajal Chakraborti; Amritlal Mandal; Sudip Das; Tapati Chakraborti
Journal:  Mol Cell Biochem       Date:  2005-12       Impact factor: 3.396

7.  Role of matrix metalloprotease-2 in oxidant activation of Ca2+ ATPase by hydrogen peroxide in pulmonary vascular smooth muscle plasma membrane.

Authors:  Malay Mandal; Sudip Das; Tapati Chakraborti; Amritlal Mandal; Sajal Chakraborti
Journal:  J Biosci       Date:  2003-03       Impact factor: 1.826

8.  PKCζ-NADPH Oxidase-PKCα Dependent Kv1.5 Phosphorylation by Endothelin-1 Modulates Nav1.5-NCX1-Cav1.2 Axis in Stimulating Ca2+ Level in Caveolae of Pulmonary Artery Smooth Muscle Cells.

Authors:  Jaganmay Sarkar; Tapati Chakraborti; Pijush Kanti Pramanik; Priyanka Ghosh; Amritlal Mandal; Sajal Chakraborti
Journal:  Cell Biochem Biophys       Date:  2020-10-23       Impact factor: 2.194

9.  alpha-Tocopherol specifically inactivates cellular protein kinase C alpha by changing its phosphorylation state.

Authors:  R Ricciarelli; A Tasinato; S Clément; N K Ozer; D Boscoboinik; A Azzi
Journal:  Biochem J       Date:  1998-08-15       Impact factor: 3.857

10.  Role of hydroxyl radical in the stimulation of arachidonic acid release caused by H2O2 in pulmonary smooth muscle cells: protective effect of anion channel blocker.

Authors:  S Chakraborti; S K Batabyal; T Chakraborti
Journal:  Mol Cell Biochem       Date:  1995-05-24       Impact factor: 3.396
  10 in total

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