Literature DB >> 8349767

Development and evaluation of a blood-free medium for determining growth curves and optimizing growth of Rochalimaea henselae.

W A Schwartzman1, C A Nesbit, E J Baron.   

Abstract

Two strains of Rochalimaea henselae were used to optimize a blood-free growth medium. Seven agar bases, four broths, and combinations of eight supplements were evaluated. Acceptable growth was achieved in media containing Fildes solution and hemin, with the best growth demonstrated in brucella broth or on brucella agar with 6 to 8% Fildes solution and 250 micrograms of hemin per ml. R. henselae utilized hemin in concentrations six times that utilized by Rochalimaea quintana. Erythrocyte membrane was necessary to achieve the full growth-promoting effect of rabbit blood.

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Year:  1993        PMID： 8349767      PMCID： PMC265650          DOI： 10.1128/jcm.31.7.1882-1885.1993

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

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Journal:  J Clin Microbiol       Date:  1992-02       Impact factor: 5.948
  5 in total
  17 in total

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9.  Experimental transmission of Bartonella henselae by the cat flea.

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