Literature DB >> 8349412

Recombinant human brain-derived neurotrophic factor (rHuBDNF). Disulfide structure and characterization of BDNF expressed in CHO cells.

C Acklin1, K Stoney, R A Rosenfeld, J A Miller, M F Rohde, M Haniu.   

Abstract

Three disulfide linkages of recombinant human brain-derived neurotrophic factor (BDNF) were determined by peptide sequence analysis and characterized by mass spectrometry. The three disulfide bonds for BDNF expressed in Chinese hamster ovary cells include Cys-13-Cys-80, Cys-58-Cys-109 and Cys-68-Cys-111, and the disulfide structure was homologous to that of nerve growth factor.

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Year:  1993        PMID： 8349412

Source DB:  PubMed          Journal:  Int J Pept Protein Res        ISSN： 0367-8377


  4 in total

Review 1.  Neurotrophins and B-cell malignancies.

Authors:  Jennifer Hillis; Michael O'Dwyer; Adrienne M Gorman
Journal:  Cell Mol Life Sci       Date:  2015-09-23       Impact factor: 9.261

2.  Human neurotrophin-3: a one-step peptide mapping method and complete disulfide characterization of the recombinant protein.

Authors:  J O Hui; J Le; V Katta; R Rosenfeld; M F Rohde; M Haniu
Journal:  J Protein Chem       Date:  1996-05

3.  Sites of iodination in recombinant human brain-derived neurotrophic factor and its effect on neurotrophic activity.

Authors:  R Rosenfeld; J S Philo; M Haniu; K Stoney; M F Rohde; G M Wu; L O Narhi; C Wong; T Boone; N N Hawkins
Journal:  Protein Sci       Date:  1993-10       Impact factor: 6.725

4.  Directed evolution of brain-derived neurotrophic factor for improved folding and expression in Saccharomyces cerevisiae.

Authors:  Michael L Burns; Thomas M Malott; Kevin J Metcalf; Benjamin J Hackel; Jonah R Chan; Eric V Shusta
Journal:  Appl Environ Microbiol       Date:  2014-07-11       Impact factor: 4.792
  4 in total

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