Effect of red palm oil on some hepatic drug-metabolizing enzymes in rats.

Red palm oil (RPO) from Elaeis guineensis is being considered for use as an edible oil in India as it is one of the richest natural sources of carotenoids. The effect of RPO on the host detoxification system, which is a vital mechanism in cancer prevention, was studied in three separate batches of Wistar/NIN inbred albino rats, and compared with controls, groundnut oil (GNO) and refined bleached deodorized palmolein oil (RBDPO). The first batch of 36 rats (12 from each group) comprised the adult males (26 wk old) of the third generation (F2b) from a multigeneration reproduction study in which three groups were fed 10% GNO or RPO or RBDPO for three generations continuously. Phase II glutathione-S-transferase (GSH-T) activity was measured in the liver cytosol of these rats after they had twice completed the process of mating, gestation, lactation and weaning, because GSH-T is one of the principal detoxifying enzymes involved in conjugating reactions of phase II metabolism. The fourth generation (F3b) weanling rats of the three groups, receiving GNO, RPO or RBDPO, were continued on the 10% oil diet for 9 wk, after which cytosolic GSH-T activity was measured. In the second experiment, eight male weanling Wistar/NIN inbred albino rats, 5 wk old, weighing 100-120 g, were fed 10% GNO, RPO or RBDPO for 4 wk in a 20% protein synthetic diet. Liver cytosolic GSH-T, reduced glutathione, microsomal total cytochrome P-450, aminopyrine N-demethylase and ethoxyresorufin-O-deethylase activity were measured to elucidate the effect of RPO on some phase I and phase II reactions. Significantly higher levels of GSH-T were observed in F2b and F3b rats given RPO than in those given GNO or RBDPO. In the second experiment, GSH-T induction was also noted, together with increased levels of reduced GSH. Phase I enzymes and total cytochrome P-450 levels were comparable between groups, indicating that no induction attributable to RPO had occurred. Thus, enhancement of one of the detoxifying phase II enzymes, in conjunction with the lack of induction of those activating phase I enzymes that are known to metabolize phenobarbitone and polycyclic aromatic hydrocarbons, suggests that RPO affords protection against chemical carcinogens, probably because of its carotenoid content.