Literature DB >> 8344524

Construction of new T vectors for direct cloning of PCR products.

Y Ichihara1, Y Kurosawa.   

Abstract

More than half of the products of PCR contain an extra A residue at the 3' end, which is the result of the template-independent activity of Taq polymerase. To facilitate cloning of the products of PCR without modification, T vectors, which have a single overhanging T residue at the 3' end, have been developed. In the present study, we constructed new T vectors which can be prepared in the laboratory by simple digestion with the restriction enzymes AspEI or Eam1 105I.

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Year:  1993        PMID： 8344524     DOI： 10.1016/0378-1119(93)90361-6

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  24 in total

1.  The PRESAT-vector: asymmetric T-vector for high-throughput screening of soluble protein domains for structural proteomics.

Authors:  Natsuko Goda; Takeshi Tenno; Hirotoshi Takasu; Hidekazu Hiroaki; Masahiro Shirakawa
Journal:  Protein Sci       Date:  2004-03       Impact factor: 6.725

2.  One-step preparation of a TA-cloning vector from a specially designed parent plasmid containing a dual lacZ gene system.

Authors:  Soo Youn Jun; Seong Jun Yoon; Sang Hyeon Kang
Journal:  Mol Biotechnol       Date:  2010-05       Impact factor: 2.695

3.  Use of base modifications in primers and amplicons to improve nucleic acids detection in the real-time snake polymerase chain reaction.

Authors:  Igor V Kutyavin
Journal:  Assay Drug Dev Technol       Date:  2010-11-04       Impact factor: 1.738

4.  Effects of mutations in DNA repair genes on formation of ribosomal DNA circles and life span in Saccharomyces cerevisiae.

Authors:  P U Park; P A Defossez; L Guarente
Journal:  Mol Cell Biol       Date:  1999-05       Impact factor: 4.272

5.  pGP-B2E, a Recombinant Compatible TA/TB-Ligation Vector for Rapid and Inexpensive Gene Cloning.

Authors:  Dongyue Li; Chao Zheng; Jie Zhou; Bin Chen; Rumeng Xu; Wenxia Yuan; Ersong Zheng; Weifang Liang; Yong Yang; Lijuan He; Jianghua Shi; Chengqi Yan; Xuming Wang; Jianping Chen
Journal:  Mol Biotechnol       Date:  2020-01       Impact factor: 2.695

6.  Does Decrease in Ribulose-1,5-Bisphosphate Carboxylase by Antisense RbcS Lead to a Higher N-Use Efficiency of Photosynthesis under Conditions of Saturating CO2 and Light in Rice Plants?

Authors:  A. Makino; T. Shimada; S. Takumi; K. Kaneko; M. Matsuoka; K. Shimamoto; H. Nakano; M. Miyao-Tokutomi; T. Mae; N. Yamamoto
Journal:  Plant Physiol       Date:  1997-06       Impact factor: 8.340

7.  Checkpoint-dependent and -independent roles of Swi3 in replication fork recovery and sister chromatid cohesion in fission yeast.

Authors:  Jordan B Rapp; Chiaki Noguchi; Mukund M Das; Lisa K Wong; Alison B Ansbach; Allyson M Holmes; Benoit Arcangioli; Eishi Noguchi
Journal:  PLoS One       Date:  2010-10-12       Impact factor: 3.240

8.  Genome-wide analyses reveal a role for peptide hormones in planarian germline development.

Authors:  James J Collins; Xiaowen Hou; Elena V Romanova; Bramwell G Lambrus; Claire M Miller; Amir Saberi; Jonathan V Sweedler; Phillip A Newmark
Journal:  PLoS Biol       Date:  2010-10-12       Impact factor: 8.029

9.  A versatile zero background T-vector system for gene cloning and functional genomics.

Authors:  Songbiao Chen; Pattavipha Songkumarn; Jianli Liu; Guo-Liang Wang
Journal:  Plant Physiol       Date:  2009-04-29       Impact factor: 8.340

10.  New approach to real-time nucleic acids detection: folding polymerase chain reaction amplicons into a secondary structure to improve cleavage of Forster resonance energy transfer probes in 5'-nuclease assays.

Authors:  Igor V Kutyavin
Journal:  Nucleic Acids Res       Date:  2009-12-07       Impact factor: 16.971
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