Literature DB >> 8344522

Cloning and sequencing of the dnaK region of Streptomyces coelicolor A3(2).

G Bucca1, C P Smith, M Alberti, G Seidita, R Passantino, A M Puglia.   

Abstract

The dnaK homologue of Streptomyces coelicolor A3(2) strain M145 has been cloned and sequenced. Nucleotide sequence analysis of a 2.5-kb region revealed an open reading frame (ORF) encoding a predicted DnaK protein of 618 amino acids (M(r) = 66,274). The dnaK coding sequence displays extreme codon bias and shows a strong preference for CGY and GGY, for Arg and Gly codons, respectively. The predicted DnaK sequence has a high Lys:Arg ratio which is not typical of streptomycete proteins. The region immediately downstream from dnaK contains an ORF for a GrpE-like protein; the predicted start codon of grpE overlaps the last two codons of dnaK, indicating that the two genes are translationally coupled. This organisation differs from that reported for other prokaryotes.

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Year:  1993        PMID： 8344522     DOI： 10.1016/0378-1119(93)90358-a

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  6 in total

1.  Regulation of the dnaK operon of Streptomyces coelicolor A3(2) is governed by HspR, an autoregulatory repressor protein.

Authors:  G Bucca; Z Hindle; C P Smith
Journal:  J Bacteriol       Date:  1997-10       Impact factor: 3.490

2.  Heat shock activation of the groESL operon of Agrobacterium tumefaciens and the regulatory roles of the inverted repeat.

Authors:  G Segal; E Z Ron
Journal:  J Bacteriol       Date:  1996-06       Impact factor: 3.490

3.  Sequencing of heat shock protein 70 (DnaK) homologs from Deinococcus proteolyticus and Thermomicrobium roseum and their integration in a protein-based phylogeny of prokaryotes.

Authors:  R S Gupta; K Bustard; M Falah; D Singh
Journal:  J Bacteriol       Date:  1997-01       Impact factor: 3.490

4.  Isolation and characterization of point mutations in the Escherichia coli grpE heat shock gene.

Authors:  B Wu; D Ang; M Snavely; C Georgopoulos
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

5.  The dnaKJ operon of Agrobacterium tumefaciens: transcriptional analysis and evidence for a new heat shock promoter.

Authors:  G Segal; E Z Ron
Journal:  J Bacteriol       Date:  1995-10       Impact factor: 3.490

6.  Identification of a Caulobacter crescentus operon encoding hrcA, involved in negatively regulating heat-inducible transcription, and the chaperone gene grpE.

Authors:  R C Roberts; C Toochinda; M Avedissian; R L Baldini; S L Gomes; L Shapiro
Journal:  J Bacteriol       Date:  1996-04       Impact factor: 3.490
  6 in total

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