Literature DB >> 8342951

cDNA cloning and expression of a soluble epoxide hydrolase from human liver.

J K Beetham1, T Tian, B D Hammock.   

Abstract

We report the cloning and expression of a cDNA that encodes a soluble epoxide hydrolase from human liver. The 2101-base clone predicts a 554-residue protein (M(r) 62,640) with an apparently imperfect peroxisomal targeting signal of Ser-Lys-Met at the carboxy terminus. The cDNA was expressed in the baculovirus system in the Spodoptera frugiperda 21 cell line. The recombinant protein was similar to soluble epoxide hydrolase isolated from human liver in terms of molecular weight, hydrolytic activity, inhibition, and immunoreactivity.

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Year:  1993        PMID： 8342951     DOI： 10.1006/abbi.1993.1411

Source DB:  PubMed          Journal:  Arch Biochem Biophys        ISSN： 0003-9861            Impact factor:   4.013


  57 in total

1.  Incorporation of piperazino functionality into 1,3-disubstituted urea as the tertiary pharmacophore affording potent inhibitors of soluble epoxide hydrolase with improved pharmacokinetic properties.

Authors:  Shao-Xu Huang; Hui-Yuan Li; Jun-Yan Liu; Christophe Morisseau; Bruce D Hammock; Ya-Qiu Long
Journal:  J Med Chem       Date:  2010-11-11       Impact factor: 7.446

2.  Evolution of a Catalytic Mechanism.

Authors:  Alissa Rauwerdink; Mark Lunzer; Titu Devamani; Bryan Jones; Joanna Mooney; Zhi-Jun Zhang; Jian-He Xu; Romas J Kazlauskas; Antony M Dean
Journal:  Mol Biol Evol       Date:  2015-12-16       Impact factor: 16.240

3.  Development of a high throughput cell-based assay for soluble epoxide hydrolase using BacMam technology.

Authors:  Wensheng Xie; Xiaoyan Tang; Quinn Lu; Robert S Ames; Steven J Ratcliffe; Hu Li
Journal:  Mol Biotechnol       Date:  2010-07       Impact factor: 2.695

4.  Human soluble epoxide hydrolase: structural basis of inhibition by 4-(3-cyclohexylureido)-carboxylic acids.

Authors:  German A Gomez; Christophe Morisseau; Bruce D Hammock; David W Christianson
Journal:  Protein Sci       Date:  2005-12-01       Impact factor: 6.725

5.  Fluorescent substrates for soluble epoxide hydrolase and application to inhibition studies.

Authors:  Paul D Jones; Nicola M Wolf; Christophe Morisseau; Paul Whetstone; Bertold Hock; Bruce D Hammock
Journal:  Anal Biochem       Date:  2005-08-01       Impact factor: 3.365

6.  Development of a high-throughput screen for soluble epoxide hydrolase inhibition.

Authors:  Nicola M Wolf; Christophe Morisseau; Paul D Jones; Bertold Hock; Bruce D Hammock
Journal:  Anal Biochem       Date:  2006-05-11       Impact factor: 3.365

7.  Design, synthesis and anti-tuberculosis activity of 1-adamantyl-3-heteroaryl ureas with improved in vitro pharmacokinetic properties.

Authors:  E Jeffrey North; Michael S Scherman; David F Bruhn; Jerrod S Scarborough; Marcus M Maddox; Victoria Jones; Anna Grzegorzewicz; Lei Yang; Tamara Hess; Christophe Morisseau; Mary Jackson; Michael R McNeil; Richard E Lee
Journal:  Bioorg Med Chem       Date:  2013-02-26       Impact factor: 3.641

8.  The N-terminal domain of mammalian soluble epoxide hydrolase is a phosphatase.

Authors:  Annette Cronin; Sherry Mowbray; Heike Dürk; Shirli Homburg; Ingrid Fleming; Beate Fisslthaler; Franz Oesch; Michael Arand
Journal:  Proc Natl Acad Sci U S A       Date:  2003-02-06       Impact factor: 11.205

9.  The soluble epoxide hydrolase encoded by EPXH2 is a bifunctional enzyme with novel lipid phosphate phosphatase activity.

Authors:  John W Newman; Christophe Morisseau; Todd R Harris; Bruce D Hammock
Journal:  Proc Natl Acad Sci U S A       Date:  2003-02-06       Impact factor: 11.205

10.  Development of On-line Liquid Chromatography-Biochemical Detection for Soluble Epoxide Hydrolase Inhibitors in Mixtures.

Authors:  David Falck; Nils Helge Schebb; Setyo Prihatiningtyas; Jiawen Zhang; Ferry Heus; Christophe Morisseau; Jeroen Kool; Bruce D Hammock; Wilfried M A Niessen
Journal:  Chromatographia       Date:  2012-11-07       Impact factor: 2.044
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