K H Cui1, P J Verma, C D Matthews. 1. Department of Obstetrics and Gynaecology, University of Adelaide, Queen Elizabeth Hospital, Woodville, Australia.
Abstract
PURPOSE: In order to determine an optimal marker to discriminate embryo injury following single-blastomere embryo biopsy, mouse embryos were examined for rates of blastocyst formation, hatching, implantation, and fetal development following single-blastomere biopsy. RESULTS: Early studies of single-blastomere biopsy (1-8 series) resulted in similar rates of blastocyst formation (P > 0.05) but a lower rate of hatching of biopsied (n = 140) versus control (nonbiopsied) (n = 145) embryos (78.6 vs 95.2%; p < 0.01). Subsequent experience (9-13 series) eliminated this difference between biopsied (n = 145) and control embryos (n = 133) (95.9 vs 94.0%; P > 0.05). Embryo transfer of hatching blastocysts of biopsied (n = 100) and nonbiopsied control (n = 100) groups resulted in equivalent rate of fetal development (70.0 vs 68.0%; p > 0.05). CONCLUSIONS: The hatching rate appeared to be a simple, sensitive, and reliable method to evaluate the single-blastomere biopsy technique.
PURPOSE: In order to determine an optimal marker to discriminate embryo injury following single-blastomere embryo biopsy, mouse embryos were examined for rates of blastocyst formation, hatching, implantation, and fetal development following single-blastomere biopsy. RESULTS: Early studies of single-blastomere biopsy (1-8 series) resulted in similar rates of blastocyst formation (P > 0.05) but a lower rate of hatching of biopsied (n = 140) versus control (nonbiopsied) (n = 145) embryos (78.6 vs 95.2%; p < 0.01). Subsequent experience (9-13 series) eliminated this difference between biopsied (n = 145) and control embryos (n = 133) (95.9 vs 94.0%; P > 0.05). Embryo transfer of hatching blastocysts of biopsied (n = 100) and nonbiopsied control (n = 100) groups resulted in equivalent rate of fetal development (70.0 vs 68.0%; p > 0.05). CONCLUSIONS: The hatching rate appeared to be a simple, sensitive, and reliable method to evaluate the single-blastomere biopsy technique.