Literature DB >> 8335643

Cloning and characterization of a Bacillus subtilis gene encoding a homolog of the 54-kilodalton subunit of mammalian signal recognition particle and Escherichia coli Ffh.

K Honda1, K Nakamura, M Nishiguchi, K Yamane.   

Abstract

By using a DNA fragment of Escherichia coli ffh as a probe, the Bacillus subtilis ffh gene was cloned. The complete nucleotide sequence of the cloned DNA revealed that it contained three open reading frames (ORFs). Their order in the region, given by the gene product, was suggested to be ORF1-Ffh-S16, according to their similarity to the gene products of E. coli, although ORF1 exhibited no significant identity with any other known proteins. The orf1 and ffh genes are organized into an operon. Genetic mapping of the ffh locus showed that the B. subtilis ffh gene is located near the pyr locus on the chromosome. The gene product of B. subtilis ffh shared 53.9 and 32.6% amino acid identity with E. coli Ffh and the canine 54-kDa subunit of signal recognition particle, respectively. Although there was low amino acid identity with the 54-kDa subunit of mammalian signal recognition particle, three GTP-binding motifs in the NH2-terminal half and amphipathic helical cores in the COOH-terminus were conserved. The depletion of ffh in B. subtilis led to growth arrest and drastic morphological changes. Furthermore, the translocation of beta-lactamase and alpha-amylase under the depleted condition was also defective.

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Year:  1993        PMID: 8335643      PMCID: PMC204942          DOI: 10.1128/jb.175.15.4885-4894.1993

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  47 in total

1.  Signal-sequence recognition by an Escherichia coli ribonucleoprotein complex.

Authors:  J Luirink; S High; H Wood; A Giner; D Tollervey; B Dobberstein
Journal:  Nature       Date:  1992-10-22       Impact factor: 49.962

2.  Topology of signal recognition particle receptor in endoplasmic reticulum membrane.

Authors:  L Lauffer; P D Garcia; R N Harkins; L Coussens; A Ullrich; P Walter
Journal:  Nature       Date:  1985 Nov 28-Dec 4       Impact factor: 49.962

3.  Genetic mapping in Bacillus subtilis.

Authors:  D Dubnau; C Goldthwaite; I Smith; J Marmur
Journal:  J Mol Biol       Date:  1967-07-14       Impact factor: 5.469

4.  Homology of 54K protein of signal-recognition particle, docking protein and two E. coli proteins with putative GTP-binding domains.

Authors:  K Römisch; J Webb; J Herz; S Prehn; R Frank; M Vingron; B Dobberstein
Journal:  Nature       Date:  1989-08-10       Impact factor: 49.962

5.  Isolation of a yeast gene, SRH1, that encodes a homologue of the 54K subunit of mammalian signal recognition particle.

Authors:  Y Amaya; A Nakano; K Ito; M Mori
Journal:  J Biochem       Date:  1990-03       Impact factor: 3.387

6.  Changes in the properties and molecular weights of Bacillus subtilis M-type and N-type alpha-amylases resulting from a spontaneous deletion.

Authors:  K Yamane; Y Hirata; T Furusato; H Yamazaki; A Nakayama
Journal:  J Biochem       Date:  1984-12       Impact factor: 3.387

7.  New shuttle vectors for Escherichia coli and Bacillus subtilis. I. Construction and characterization of plasmid pHY460 with twelve unique cloning sites.

Authors:  H Ishiwa; N Tsuchida
Journal:  Gene       Date:  1984-12       Impact factor: 3.688

8.  The nucleotide sequence of an Escherichia coli operon containing genes for the tRNA(m1G)methyltransferase, the ribosomal proteins S16 and L19 and a 21-K polypeptide.

Authors:  A S Byström; K J Hjalmarsson; P M Wikström; G R Björk
Journal:  EMBO J       Date:  1983       Impact factor: 11.598

9.  The signal sequence interacts with the methionine-rich domain of the 54-kD protein of signal recognition particle.

Authors:  S High; B Dobberstein
Journal:  J Cell Biol       Date:  1991-04       Impact factor: 10.539

10.  Direct probing of the interaction between the signal sequence of nascent preprolactin and the signal recognition particle by specific cross-linking.

Authors:  M Wiedmann; T V Kurzchalia; H Bielka; T A Rapoport
Journal:  J Cell Biol       Date:  1987-02       Impact factor: 10.539

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  22 in total

1.  Characterization of the sat operon in Streptococcus mutans: evidence for a role of Ffh in acid tolerance.

Authors:  B H Kremer; M van der Kraan; P J Crowley; I R Hamilton; L J Brady; A S Bleiweis
Journal:  J Bacteriol       Date:  2001-04       Impact factor: 3.490

2.  Streptococcal viability and diminished stress tolerance in mutants lacking the signal recognition particle pathway or YidC2.

Authors:  Adnan Hasona; Paula J Crowley; Celine M Levesque; Richard W Mair; Dennis G Cvitkovitch; Arnold S Bleiweis; L Jeannine Brady
Journal:  Proc Natl Acad Sci U S A       Date:  2005-11-17       Impact factor: 11.205

3.  YlxM is a newly identified accessory protein that influences the function of signal recognition particle pathway components in Streptococcus mutans.

Authors:  Matthew L Williams; Paula J Crowley; Adnan Hasona; L Jeannine Brady
Journal:  J Bacteriol       Date:  2014-03-21       Impact factor: 3.490

4.  The cyanobacterial genome contains a single copy of the ffh gene encoding a homologue of the 54 kDa subunit of signal recognition particle.

Authors:  J C Packer; C J Howe
Journal:  Plant Mol Biol       Date:  1996-02       Impact factor: 4.076

5.  Biochemical characterization of the essential GTP-binding protein Obg of Bacillus subtilis.

Authors:  K M Welsh; K A Trach; C Folger; J A Hoch
Journal:  J Bacteriol       Date:  1994-12       Impact factor: 3.490

6.  Insertional mutagenesis and recovery of interrupted genes of Streptococcus mutans by using transposon Tn917: preliminary characterization of mutants displaying acid sensitivity and nutritional requirements.

Authors:  J A Gutierrez; P J Crowley; D P Brown; J D Hillman; P Youngman; A S Bleiweis
Journal:  J Bacteriol       Date:  1996-07       Impact factor: 3.490

7.  Identification of ZipA, a signal recognition particle-dependent protein from Neisseria gonorrhoeae.

Authors:  Ying Du; Cindy Grove Arvidson
Journal:  J Bacteriol       Date:  2003-04       Impact factor: 3.490

8.  The Srp54 GTPase is essential for protein export in the fission yeast Schizosaccharomyces pombe.

Authors:  S M Althoff; S W Stevens; J A Wise
Journal:  Mol Cell Biol       Date:  1994-12       Impact factor: 4.272

9.  Structural and functional characterisation of the signal recognition particle-specific 54 kDa protein (SRP54) of tomato.

Authors:  S Krolkiewicz; H L Sänger; U Niesbach-Klösgen
Journal:  Mol Gen Genet       Date:  1994-12-01

10.  FlhF, the third signal recognition particle-GTPase of Bacillus subtilis, is dispensable for protein secretion.

Authors:  Geeske Zanen; Haike Antelmann; Helga Westers; Michael Hecker; Jan Maarten van Dijl; Wim J Quax
Journal:  J Bacteriol       Date:  2004-09       Impact factor: 3.490

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