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Literature DB >> 8335088

Phorbol ester markedly increases the sensitivity of intestinal epithelial cells to 1,25-dihydroxyvitamin D3.

H J Armbrecht¹, T L Hodam, M A Boltz, M L Chen.

Abstract

We have used a clonal intestinal epithelial cell line (IEC-18) to study the mechanism of action of 1,25-dihydroxyvitamin D3 (1,25(OH)2D) in vitro. 1,25(OH)2D(10(-7) M) elevated by over 10-fold the mRNA levels for the cytochrome P450 component (P450cc24) of the 1,25(OH)2D-24-hydroxylase. Increased P450cc24 mRNA levels were detectable at 6 h and peaked at 36 h. Below a concentration of 10(-7) M, 1,25(OH)2D had almost no effect. However, addition of phorbol ester for 2 h made the intestine responsive to 1,25(OH)2D concentrations as low as 10(-9) M.

Entities: Chemical Gene

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Year: 1993 PMID： 8335088 DOI： 10.1016/0014-5793(93)81028-x

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

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Cited

7 in total

1. Stat1-vitamin D receptor interactions antagonize 1,25-dihydroxyvitamin D transcriptional activity and enhance stat1-mediated transcription.

Authors: Marcos Vidal; Chilakamarti V Ramana; Adriana S Dusso
Journal: Mol Cell Biol Date: 2002-04 Impact factor: 4.272

2. Molecular action of 1,25-dihydroxyvitamin D3 and phorbol ester on the activation of the rat cytochrome P450C24 (CYP24) promoter: role of MAP kinase activities and identification of an important transcription factor binding site.

Authors: Barbara K Nutchey; Josef S Kaplan; Prem P Dwivedi; John L Omdahl; Antonio Ferrante; Brian K May; Charles S T Hii
Journal: Biochem J Date: 2005-08-01 Impact factor: 3.857

3. Phorbol esters enhance 1α,25-dihydroxyvitamin D3-regulated 25-hydroxyvitamin D-24-hydroxylase (CYP24A1) gene expression through ERK-mediated phosphorylation of specific protein 3 (Sp3) in Caco-2 cells.

Authors: Yan Jiang; James C Fleet
Journal: Mol Cell Endocrinol Date: 2012-03-28 Impact factor: 4.102

4. Effect of phorbol 12-myristate 13-acetate activated signaling pathways on 1α, 25 dihydroxyvitamin D3 regulated human 25-hydroxyvitamin D3 24-hydroxylase gene expression in differentiated Caco-2 cells.

Authors: Yan Jiang; James C Fleet
Journal: J Cell Biochem Date: 2012-05 Impact factor: 4.429

Phorbol ester markedly increases the sensitivity of intestinal epithelial cells to 1,25-dihydroxyvitamin D3.

1. Stat1-vitamin D receptor interactions antagonize 1,25-dihydroxyvitamin D transcriptional activity and enhance stat1-mediated transcription.

2. Molecular action of 1,25-dihydroxyvitamin D3 and phorbol ester on the activation of the rat cytochrome P450C24 (CYP24) promoter: role of MAP kinase activities and identification of an important transcription factor binding site.

3. Phorbol esters enhance 1α,25-dihydroxyvitamin D3-regulated 25-hydroxyvitamin D-24-hydroxylase (CYP24A1) gene expression through ERK-mediated phosphorylation of specific protein 3 (Sp3) in Caco-2 cells.

4. Effect of phorbol 12-myristate 13-acetate activated signaling pathways on 1α, 25 dihydroxyvitamin D3 regulated human 25-hydroxyvitamin D3 24-hydroxylase gene expression in differentiated Caco-2 cells.

5. Development of a vitamin D-responsive organ culture system for adult and old rat intestine.

Review 6. Genomic mechanisms involved in the pleiotropic actions of 1,25-dihydroxyvitamin D3.

Review 7. Mechanistic Effects of Calcitriol in Cancer Biology.