Literature DB >> 8334308

In vitro fusion of endocytic vesicles is inhibited by cyclin A-cdc2 kinase.

P G Woodman1, J P Adamczewski, T Hunt, G Warren.   

Abstract

Receptor-mediated endocytosis and recycling are inhibited in mitotic mammalian cells, and previous studies have shown that inhibition of endocytic vesicle fusion in vitro occurs via cyclin B-cdc2 kinase. To test for the ability of cyclin A-cdc2 kinase to inhibit endocytic vesicle fusion, we employed recombinant cyclin A proteins. Addition of cyclin A to interphase extracts activated a histone kinase and markedly reduced the efficiency of endocytic vesicle fusion. By a number of criteria, inhibition of fusion was shown to be due to the action of cyclin A, via the mitosis-specific cdc2 kinase, and not an indirect effect through cyclin B. Two-stage incubations were used to demonstrate that at least one target of cyclin A-cdc2 kinase is a cytosolic component of the fusion apparatus. Reconstitution experiments showed that this component was also modified in mitotic cytosols and was unaffected by N-ethyl maleimide treatment.

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Year:  1993        PMID: 8334308      PMCID: PMC300957          DOI: 10.1091/mbc.4.5.541

Source DB:  PubMed          Journal:  Mol Biol Cell        ISSN: 1059-1524            Impact factor:   4.138


  74 in total

1.  Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits.

Authors:  H Kobayashi; E Stewart; R Poon; J P Adamczewski; J Gannon; T Hunt
Journal:  Mol Biol Cell       Date:  1992-11       Impact factor: 4.138

2.  Inhibition of membrane fusion in vitro via cyclin B but not cyclin A.

Authors:  L Thomas; P R Clarke; M Pagano; J Gruenberg
Journal:  J Biol Chem       Date:  1992-03-25       Impact factor: 5.157

3.  Activation of human cyclin-dependent kinases in vitro.

Authors:  D Desai; Y Gu; D O Morgan
Journal:  Mol Biol Cell       Date:  1992-05       Impact factor: 4.138

4.  Sphingolipid transport in mitotic HeLa cells.

Authors:  R N Collins; G Warren
Journal:  J Biol Chem       Date:  1992-12-05       Impact factor: 5.157

5.  Cyclin A-cdc2 kinase does not trigger but delays cyclin degradation in interphase extracts of amphibian eggs.

Authors:  T Lorca; J C Labbé; A Devault; D Fesquet; U Strausfeld; J Nilsson; P A Nygren; M Uhlen; J C Cavadore; M Dorée
Journal:  J Cell Sci       Date:  1992-05       Impact factor: 5.285

6.  Cyclin A- and cyclin B-dependent protein kinases are regulated by different mechanisms in Xenopus egg extracts.

Authors:  P R Clarke; D Leiss; M Pagano; E Karsenti
Journal:  EMBO J       Date:  1992-05       Impact factor: 11.598

7.  Synergistic action of Drosophila cyclins A and B during the G2-M transition.

Authors:  J A Knoblich; C F Lehner
Journal:  EMBO J       Date:  1993-01       Impact factor: 11.598

8.  Control of microtubule dynamics and length by cyclin A- and cyclin B-dependent kinases in Xenopus egg extracts.

Authors:  F Verde; M Dogterom; E Stelzer; E Karsenti; S Leibler
Journal:  J Cell Biol       Date:  1992-09       Impact factor: 10.539

9.  Requirement for p34cdc2 kinase is restricted to mitosis in the mammalian cdc2 mutant FT210.

Authors:  J R Hamaguchi; R A Tobey; J Pines; H A Crissman; T Hunter; E M Bradbury
Journal:  J Cell Biol       Date:  1992-06       Impact factor: 10.539

10.  Cyclin A potentiates maturation-promoting factor activation in the early Xenopus embryo via inhibition of the tyrosine kinase that phosphorylates cdc2.

Authors:  A Devault; D Fesquet; J C Cavadore; A M Garrigues; J C Labbé; T Lorca; A Picard; M Philippe; M Dorée
Journal:  J Cell Biol       Date:  1992-09       Impact factor: 10.539

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  10 in total

1.  Cleavage of rabaptin-5 blocks endosome fusion during apoptosis.

Authors:  S C Cosulich; H Horiuchi; M Zerial; P R Clarke; P G Woodman
Journal:  EMBO J       Date:  1997-10-15       Impact factor: 11.598

2.  Breaking up is hard to do - membrane traffic in cytokinesis.

Authors:  Rytis Prekeris; Gwyn W Gould
Journal:  J Cell Sci       Date:  2008-05-15       Impact factor: 5.285

Review 3.  Cyclins and cyclin-dependent kinases: a biochemical view.

Authors:  J Pines
Journal:  Biochem J       Date:  1995-06-15       Impact factor: 3.857

4.  Multiple N-ethylmaleimide-sensitive components are required for endosomal vesicle fusion.

Authors:  L Rodriguez; C J Stirling; P G Woodman
Journal:  Mol Biol Cell       Date:  1994-07       Impact factor: 4.138

5.  Clathrin-mediated endocytosis is inhibited during mitosis.

Authors:  Andrew B Fielding; Anna K Willox; Emmanuel Okeke; Stephen J Royle
Journal:  Proc Natl Acad Sci U S A       Date:  2012-04-09       Impact factor: 11.205

6.  A role for tubular networks and a COP I-independent pathway in the mitotic fragmentation of Golgi stacks in a cell-free system.

Authors:  T Misteli; G Warren
Journal:  J Cell Biol       Date:  1995-09       Impact factor: 10.539

7.  COP-coated vesicles are involved in the mitotic fragmentation of Golgi stacks in a cell-free system.

Authors:  T Misteli; G Warren
Journal:  J Cell Biol       Date:  1994-04       Impact factor: 10.539

8.  Cyclin A2 localises in the cytoplasm at the S/G2 transition to activate PLK1.

Authors:  Helena Silva Cascales; Kamila Burdova; Anna Middleton; Vladislav Kuzin; Erik Müllers; Henriette Stoy; Laura Baranello; Libor Macurek; Arne Lindqvist
Journal:  Life Sci Alliance       Date:  2021-01-05

9.  Human cyclins B1 and B2 are localized to strikingly different structures: B1 to microtubules, B2 primarily to the Golgi apparatus.

Authors:  M Jackman; M Firth; J Pines
Journal:  EMBO J       Date:  1995-04-18       Impact factor: 11.598

10.  Cell cycle-dependent regulation of the phosphorylation and metabolism of the Alzheimer amyloid precursor protein.

Authors:  T Suzuki; M Oishi; D R Marshak; A J Czernik; A C Nairn; P Greengard
Journal:  EMBO J       Date:  1994-03-01       Impact factor: 11.598

  10 in total

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