| Literature DB >> 833423 |
B P Lane, J Elias, E Drummond.
Abstract
The ultrastructural distribution of alpha-actinin in skeletal myocytes was studied by a sensitive method that permits localization of antigenic proteins in intact fixed cells rather than in glycerol estracted tissues or isolated cell fractions. Binding to the Z line of specific antiserum produced in rabbits immunized with highly purified alpha-actinin was demonstrated by deposition at this site of a reaction product of horseradish peroxidase that had been coupled to antigen-binding fragments of sheep anti-rabbit gamma globulin.Entities:
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Year: 1977 PMID: 833423 DOI: 10.1177/25.1.833423
Source DB: PubMed Journal: J Histochem Cytochem ISSN: 0022-1554 Impact factor: 2.479