Literature DB >> 8332517

An alternative hot start technique for PCR in small volumes using beads of wax-embedded reaction components dried in trehalose.

S Kaijalainen1, P J Karhunen, K Lalu, K Lindström.   

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Year:  1993        PMID： 8332517      PMCID： PMC309708          DOI： 10.1093/nar/21.12.2959

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


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  3 in total

1.  Extraordinary stability of enzymes dried in trehalose: simplified molecular biology.

Authors:  C Colaço; S Sen; M Thangavelu; S Pinder; B Roser
Journal:  Biotechnology (N Y)       Date:  1992-09

2.  Polymerase chain reaction amplification products separated on rehydratable polyacrylamide gels and stained with silver.

Authors:  R C Allen; G Graves; B Budowle
Journal:  Biotechniques       Date:  1989 Jul-Aug       Impact factor: 1.993

3.  DNA typing and genetic mapping with trimeric and tetrameric tandem repeats.

Authors:  A Edwards; A Civitello; H A Hammond; C T Caskey
Journal:  Am J Hum Genet       Date:  1991-10       Impact factor: 11.025
  3 in total
  9 in total

1.  Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR.

Authors:  Milko B Kermekchiev; Anatoly Tzekov; Wayne M Barnes
Journal:  Nucleic Acids Res       Date:  2003-11-01       Impact factor: 16.971

2.  An Alternative Hot Start PCR Method Using a Nuclease-Deficient ExoIII from Escherichia coli.

Authors:  Shuhong Lu; Xuesong Zhang; Kaiying Chen; Bingbin Xie; Dapeng Shan; Yulong Shen; Zhuo Li
Journal:  Mol Biotechnol       Date:  2019-12       Impact factor: 2.695

3.  3'-Protected 2'-deoxynucleoside 5'-triphosphates as a tool for heat-triggered activation of polymerase chain reaction.

Authors:  Inna Koukhareva; Alexandre Lebedev
Journal:  Anal Chem       Date:  2009-06-15       Impact factor: 6.986

4.  Reagent integration and controlled release for multiplexed nucleic acid testing in disposable thermoplastic 2D microwell arrays.

Authors:  S Padmanabhan; A Sposito; M Yeh; M Everitt; I White; D L DeVoe
Journal:  Biomicrofluidics       Date:  2021-01-15       Impact factor: 2.800

5.  Adaptation of a visualized loop-mediated isothermal amplification technique for field detection of Plasmodium vivax infection.

Authors:  Zhi-Yong Tao; Hua-Yun Zhou; Hui Xia; Sui Xu; Han-Wu Zhu; Richard L Culleton; Eun-Taek Han; Feng Lu; Qiang Fang; Ya-Ping Gu; Yao-Bao Liu; Guo-Ding Zhu; Wei-Ming Wang; Ju-Lin Li; Jun Cao; Qi Gao
Journal:  Parasit Vectors       Date:  2011-06-21       Impact factor: 3.876

6.  Characterization of family IV UDG from Aeropyrum pernix and its application in hot-start PCR by family B DNA polymerase.

Authors:  Xi-Peng Liu; Jian-Hua Liu
Journal:  PLoS One       Date:  2011-11-08       Impact factor: 3.240

7.  Hot start PCR with heat-activatable primers: a novel approach for improved PCR performance.

Authors:  Alexandre V Lebedev; Natasha Paul; Joyclyn Yee; Victor A Timoshchuk; Jonathan Shum; Kei Miyagi; Jack Kellum; Richard I Hogrefe; Gerald Zon
Journal:  Nucleic Acids Res       Date:  2008-09-16       Impact factor: 16.971

8.  Covalent modification of primers improves PCR amplification specificity and yield.

Authors:  Nancy J Schoenbrunner; Amar P Gupta; Karen K Y Young; Stephen G Will
Journal:  Biol Methods Protoc       Date:  2017-11-21

9.  Single-tube collection and nucleic acid analysis of clinical samples for SARS-CoV-2 saliva testing.

Authors:  Kyle H Cole; Alexis Bouin; Caila Ruiz; Bert L Semler; Matthew A Inlay; Andrej Lupták
Journal:  Sci Rep       Date:  2022-03-10       Impact factor: 4.379
  9 in total

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